scispace - formally typeset
Journal ArticleDOI

Characterization of a thermostable, CaCl2-activated and raw-starch hydrolyzing alpha-amylase from Bacillus licheniformis AT70: Production under solid state fermentation by utilizing agricultural wastes

Reads0
Chats0
TLDR
In this paper, B. licheniformis AT70 which produced a thermophilic, raw-starch degrading alpha-amylase was isolated from Gorooh hot springs in Kerman province.
Abstract
B. licheniformis AT70 which produced a thermophilic, raw-starch degrading alpha-amylase was isolated from Gorooh hot springs in Kerman province. Maximum production of AT70 alpha-amylase was obtained in the presence of starch (as a carbon source) and ammonium chloride (as a nitrogen source) with 388 and 329 U/ml enzyme yield, respectively. SSF was also carried out using various agricultural and kitchen wastes and results showed that the maximum yield of AT70 alpha-amylase production was obtained by date waste and wheat bran, respectively (10%, w/v). The thermal stability of the AT70 alpha-amylase was increased about 2.5 folds at 60 °C. AT70 alpha-amylase showed the maximum activity at 1.5 M NaCl by 42% and local detergent Shooma enhanced the alpha-amylase activity about 34%, compared to control. Furthermore, AT70 alpha-amylase exhibited remarkable hydrolytic activity in a range of 14–20% (w/v) of raw corn starch at 55 °C. These results indicated that AT70 alpha-amylase has great potential applications for the raw-starch degrading.

read more

Citations
More filters
Journal ArticleDOI

Recent developments and innovations in solid state fermentation

TL;DR: In this paper, an overview of solid-state fermentation (SSF), focusing on its applications, mainly of the very recent papers of the last five years, is presented, which comprises the most important developed processes concerning the production of enzymes, biopulping processes, and traditional processes, for food fermentation, such as Chinese daqu and koji, and industrial important biomolecules such as organic acids, pigments, phenolic compounds, aromas and biosorbents.
Journal ArticleDOI

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

TL;DR: In this article, a review of the management of solid wastes using Solid State Fermentation (SSF), with regard to its current application, advantages and challenges, downstream processing in SSF, economic viewpoint, and future perspectives is presented.
Journal ArticleDOI

Biochemical characterization of a novel cold-active, halophilic and organic solvent-tolerant lipase from B. licheniformis KM12 with potential application for biodiesel production.

TL;DR: These unique properties of KM12 lipase make it talented as a potential biocatalyst for biodiesel production, especially in polar organic solvents.
Journal ArticleDOI

Development of thermostable amylase enzyme from Bacillus cereus for potential antibiofilm activity.

TL;DR: Findings suggested that marine bacterial derived amylase enzyme could be developed as potential antibiofilm agent.
References
More filters
Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

TL;DR: It was found that treatment of gels with dithiothreitol prior to impregnation with silver nitrate results in more reproducible staining patterns that are also qualitatively similar to those obtained with Coomassie blue.
Book ChapterDOI

[17] Amylases, α and β

Journal ArticleDOI

Microbial heavy-metal resistance

TL;DR: This review describes the workings of known metal-resistance systems in microorganisms and the transport of the 17 most important (heavy metal) elements is compared.
Related Papers (5)