Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure
Hiroshi Tsujibo,Hideyuki Orikoshi,Kayoko Shiotani,Miyuki Hayashi,Junko Umeda,Katsushiro Miyamoto,Chiaki Imada,Yoshiro Okami,Yoshihiko Inamori +8 more
TLDR
To evaluate the role of the domain, the hybrid gene that directs the synthesis of the fusion protein with glutathione S-transferase activity was constructed, indicating that the N-terminal domain of ChiC constitutes an independent chitin-binding domain.Abstract:
One of the chitinase genes of Alteromonas sp. strain O-7, the chitinase C-encoding gene (chiC), was cloned, and the nucleotide sequence was determined. An open reading frame coded for a protein of 430 amino acids with a predicted molecular mass of 46,680 Da. Alignment of the deduced amino acid sequence demonstrated that ChiC contained three functional domains, the N-terminal domain, a fibronectin type III-like domain, and a catalytic domain. The N-terminal domain (59 amino acids) was similar to that found in the C-terminal extension of ChiA (50 amino acids) of this strain and furthermore showed significant sequence homology to the regions found in several chitinases and cellulases. Thus, to evaluate the role of the domain, we constructed the hybrid gene that directs the synthesis of the fusion protein with glutathione S-transferase activity. Both the fusion protein and the N-terminal domain itself bound to chitin, indicating that the N-terminal domain of ChiC constitutes an independent chitin-binding domain.read more
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Chitinolytic Activity in Chromobacterium violaceum: Substrate Analysis and Regulation by Quorum Sensing
Leonid Chernin,Michael K. Winson,Jacquelyn M. Thompson,Shoshan Haran,Barrie W. Bycroft,Ilan Chet,Paul Williams,Gordon S. A. B. Stewart +7 more
TL;DR: This study shows that C. violaceum produces a set of chitinolytic enzymes whose production is regulated by HHL, and finds that a pleiotropic mini-Tn5 mutant of C. Violaceum that is defective in HHL production and other quorum-sensing-regulated factors was found to be completely deficient in chitinase activity.
Journal ArticleDOI
A conserved domain in arthropod cuticular proteins binds chitin.
John E Rebers,Judith H. Willis +1 more
TL;DR: It is shown that an extended form of the R&R consensus from proteins of hard cuticles is necessary and sufficient for chitin binding and that arthropods have two distinct classes of chitIn binding proteins, those with the chITin-binding domain found in lectins, chit inases and peritrophic membranes (cysCBD) and those without the cuticular protein ch itin- binding domain (non-cysBBD).
Journal ArticleDOI
Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung
TL;DR: The output of type II secretion is greater in magnitude than previously appreciated and includes previously undescribed proteins, and data indicate that an enzyme with chitinase activity can promote infection of a mammalian host.
Journal ArticleDOI
Chitinases A, B, and C1 of Serratia marcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation.
Kazushi Suzuki,Kazushi Suzuki,Noriko Sugawara,Megumi Suzuki,Taku Uchiyama,Fuminori Katouno,Naoki Nikaidou,Takeshi Watanabe +7 more
TL;DR: In this article, the individual roles of the chitinases from Serratia marcescens 2170 were studied and a broad pH optimum was found for chitinolytic activity between pH 4 and 10, and the sites attacked by ChiA on the substrate were different from those by either ChiB or ChiC1.
Journal ArticleDOI
Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.
Masayuki Hashimoto,Takahisa Ikegami,Shizuka Seino,Nobuhumi Ohuchi,Harumi Fukada,Junji Sugiyama,Masahiro Shirakawa,Takeshi Watanabe +7 more
TL;DR: Observations suggest that ChBD(ChiA1) recognizes a structure which is present in insoluble or crystalline chitin but not in chito-oligosaccharides or in soluble derivatives of chitIn, and exhibited binding activity over a wide range of pHs.
References
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