Journal ArticleDOI
Characterization of human sperm plasma membrane: Glycolipids and polypeptides
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TLDR
Glycolipid analysis of the plasma membrane revealed a three-fold enrichment in gangliosides: GM3 and GD1a/GD1b and neutral glycolipids: globoside and sulfatide as compared to that of whole human sperm.Abstract:
The plasma membranes from ejaculated human spermatozoa were removed by nitrogen cavitation (600 PSI for 10 min) and isolated by centrifugation followed by a discontinuous sucrose density gradient centrifugation. Glycolipid analysis of the plasma membrane revealed a three-fold enrichment in gangliosides: GM3 and GD1a/GD1b and neutral glycolipids: globoside and sulfatide as compared to that of whole human sperm. Two dimensional electrophoresis of human sperm plasma membranes revealed about 75 polypeptides. Several of these polypeptides were similar in migration and in display of shape and color to that found in boar sperm plasma membranes.read more
Citations
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Journal ArticleDOI
Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa.
TL;DR: Novel isoforms of actin, beta-tubulin, PH-20, and several phosphotyrosine-containing proteins were identified in human sperm, which aided the identification of sperm-coating proteins acquired from secretions of the accessory sex organs.
Journal ArticleDOI
Lipid domains in the ram sperm plasma membrane demonstrated by differential scanning calorimetry
TL;DR: In this article, the authors used differential scanning calorimetry to show that the anterior region of the head of a ram sperm exhibits at least two major endothermic transitions, one centered at approximately 26 degrees C and one centred at approximately 60 degrees C, consistent with gel-to-fluid transitions in model membranes.
Journal ArticleDOI
Localization of Low-Density Detergent-Resistant Membrane Proteins in Intact and Acrosome-Reacted Mouse Sperm
TL;DR: Taking into consideration the requirement of the acrosome reaction for sperm to become fusogenic, these results suggest that membrane raft dynamics may have a role in sperm-egg membrane interaction.
Journal ArticleDOI
Proteomics of semen and its constituents
TL;DR: This review examines the application of proteomic methods to the study of spermatozoa and seminal fluid and highlights some of the unique challenges associated with the collection, fractionation, and analysis of this fluid.
Book ChapterDOI
CHAPTER 1 – The Spermatozoon
TL;DR: This chapter focuses on the novel structural features of the spermatozoon, their composition and assembly, and the mechanisms regulating their function.
References
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of basic as well as acidic proteins
TL;DR: This paper describes an alternate procedure for the first dimension which, unlike isoelectric focusing, resolves basic as well as acidic proteins, and involves a short time of electrophoresis toward the cathode and separates most proteins according to their isoelection points.
Journal ArticleDOI
CHROMATOGRAPHlC SEPARATION OF HUMAN BRAIN GANGLIOSIDES
TL;DR: Chromatographic methods for the quantitative isolation and separation of human brain gangliosides are described and it is shown that predominantly the gangLiosides have a common basic structure : N-acyl-sphingosine-glucose-Galactose-galactoseN-acetylgalactosamine-gal actose to which one or more molecules of N-acetelneuraininic acid are bound.
Book
A manual of paper chromatography and paper electrophoresis.
TL;DR: Find the secret to improve the quality of life by reading this a manual of paper chromatography and paper electrophoresis and make the words as your good value to your life.