Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain.
Fernanda de Pace,Jacqueline Boldrin de Paiva,Gerson Nakazato,Marcelo Lancellotti,Marcelo Palma Sircili,Eliana Guedes Stehling,Wanderley Dias da Silveira,Vanessa Sperandio +7 more
TLDR
The results suggest that IcmF in APEC may play a role in disease, and potentially also in the epidemiological spread of this pathogen through enhancement of biofilm formation.Abstract:
The intracellular multiplication factor (IcmF) protein is a component of the recently described type VI secretion system (T6SS). IcmF has been shown to be required for intra-macrophage replication and inhibition of phagosome–lysosome fusion in Legionella pneumophila. In Vibrio cholerae it is involved in motility, adherence and conjugation. Given that we previously reported that two T6SS genes (hcp and clpV) contribute to the pathogenesis of a septicaemic strain (SEPT362) of avian pathogenic Escherichia coli (APEC), we investigated the function of IcmF in this strain. Further elucidation of the virulence mechanisms of APEC is important because this pathogen is responsible for financial losses in the poultry industry, and is closely related to human extraintestinal pathogenic E. coli (ExPEC) strains, representing a potential zoonotic risk, as well as serving as a reservoir of virulence genes. Here we show that an APEC icmF mutant has decreased adherence to and invasion of epithelial cells, as well as decreased intra-macrophage survival. The icmF mutant is also defective for biofilm formation on abiotic surfaces. Additionally, expression of the flagella operon is decreased in the icmF mutant, leading to decreased motility. The combination of these phenotypes culminates in this mutant being altered for infection in chicks. These results suggest that IcmF in APEC may play a role in disease, and potentially also in the epidemiological spread of this pathogen through enhancement of biofilm formation.read more
Citations
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Journal ArticleDOI
Diseases of Poultry
TL;DR: In this article, Gray has written a book on diseases of poultry, Diseases of Poultry Their Aetiology, Diagnosis, Treatment and Control; with a Section on the Normal Anatomy and Physiology of the Fowl.
Journal ArticleDOI
Structure and regulation of the type VI secretion system.
TL;DR: This review discusses recent progress toward characterizing T6S structure and regulation, a contact-dependent pathway with specific cellular targets, and how these complexes interact to promote effector secretion and cell targeting.
Journal ArticleDOI
Type VI secretion and anti-host effectors
TL;DR: The type VI secretion system (T6SS) mediates bacterial antagonism in various environments including eukaryotic niches, such as the gut, and effectors impede host cell functions and promote immune evasion, thereby enabling successful infection.
Journal ArticleDOI
The Type VI secretion system - a widespread and versatile cell targeting system.
TL;DR: Although it is clear that T6SSs can be deployed as versatile weapons to compete with other bacteria or attack simple or higher eukaryotes, much remains to be determined about this intriguing system.
Journal ArticleDOI
Type VI secretion systems in plant‐associated bacteria
TL;DR: The even distribution of the system among commensal and pathogenic phytobacteria suggests that the T6SS provides fitness and colonization advantages in planta and that the role of the T 6SS is not restricted to virulence.
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Diseases of Poultry
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