Journal ArticleDOI
Characterization of NDM-7 Carbapenemase-Producing Escherichia coli Isolates in the Arabian Peninsula.
Tibor Pál,Akela Ghazawi,Dania Darwish,Laura Villa,Alessandra Carattoli,Rayhan Hashmey,Zulfa O. AlDeesi,Wafaa Jamal,Vincent O. Rotimi,Amina Al-Jardani,Seif Al-Abri,Agnes Sonnevend +11 more
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TLDR
Findings show that IncX3 type plasmids play an important role in the spread of the currently rare NDM-7 variant in the Arabian Peninsula, and this association of blaNDM-7 with the Incx3-type plasmid is particularly worrisome.Abstract:
Aim: The purpose of this study was to characterize the New Delhi metallo-beta lactamase (NDM)-7-producing Enterobacteriaceae isolated in the Arabian Peninsula. Methods: Enterobacteriaceae identified to carry blaNDM-7 in a collection of 157 NDM-producing isolates from Kuwait, Oman, Saudi Arabia, and the United Arab Emirates (UAE) were investigated for their antibiotic and disinfectant susceptibility, and resistance gene content. The virulence profile, phylogenetic and sequence types of the isolates were also determined. The plasmids carrying the blaNDM-7 were transferred, and their complete nucleotide sequence was determined. Results: Four NDM-7-producing Escherichia coli isolated in Kuwait, Oman, and the UAE, respectively, were identified. They were clonally unrelated, carried a few virulence determinants only, and belonged to clonal complexes CC10 and CC23, or ST448. They were all multi-drug resistant but remained susceptible to fosfomycin, tigecycline, and colistin. In all isolates, blaNDM-7 was located...read more
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Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae
Marta Rozwandowicz,Michael S.M. Brouwer,Jennie Fischer,Jaap A. Wagenaar,Bruno Gonzalez-Zorn,Beatriz Guerra,Dik Mevius,Joost Hordijk +7 more
TL;DR: The purpose of this review is to provide an overview of all known AMR-related plasmid families in Enterobacteriaceae, the resistance genes they carry and their geographical distribution.
Journal ArticleDOI
Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach.
TL;DR: The need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries is re-emphasized.
Journal ArticleDOI
Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide.
Shaimaa F. Mouftah,Tibor Pál,Dania Darwish,Akela Ghazawi,Laura Villa,Alessandra Carattoli,Agnes Sonnevend,Agnes Sonnevend +7 more
TL;DR: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.
Journal ArticleDOI
Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital.
TL;DR: An increased number of carbapenem-resistant NDM producing enterobacteriaceae isolates recovered from NICU which is alarming signal for health workers and policy makers is alarmed signal and it is utmost important to think about infection control measures.
Journal ArticleDOI
Characterization of NDM-Encoding Plasmids From Enterobacteriaceae Recovered From Czech Hospitals
Veronika Paskova,Matej Medvecky,Anna Skalova,Katerina Chudejova,Ibrahim Bitar,Vladislav Jakubu,Tamara Bergerová,Helena Zemlickova,Costas C. Papagiannitsis,Jaroslav Hrabak +9 more
TL;DR: Analysis of plasmid sequences revealed that IncX3 plasmids have played a major role in the dissemination of blaNDM-like genes in Czech hospitals, and in combination with further evolvement of NDM-like-encoding MDR plasids through reshuffling, N DM-like producers pose an important public threat.
References
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Journal ArticleDOI
In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing
Alessandra Carattoli,Ea Zankari,Aurora García-Fernández,Mette Voldby Larsen,Ole Lund,Laura Villa,Frank Møller Aarestrup,Henrik Hasman +7 more
TL;DR: Two easy-to-use Web tools for in silico detection and characterization of whole-genome sequence (WGS) and whole-plasmid sequence data from members of the family Enterobacteriaceae are designed and developed.
Journal ArticleDOI
Rapid and simple determination of the Escherichia coli phylogenetic group.
TL;DR: In this paper, a simple and rapid phylogenetic grouping technique based on triplex PCR was proposed, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
Journal ArticleDOI
Sex and virulence in Escherichia coli: an evolutionary perspective
Thierry Wirth,Daniel Falush,Ruiting Lan,Frances M. Colles,Patience Mensa,Lothar H. Wieler,Helge Karch,Peter R. Reeves,Martin C. J. Maiden,Howard Ochman,Mark Achtman +10 more
TL;DR: The evolution of virulence is linked to bacterial sex because rates of evolution have accelerated in pathogenic lineages, culminating in highly virulent organisms whose genomic contents are altered frequently by increased rates of homologous recombination.
Journal ArticleDOI
Extended Virulence Genotypes of Escherichia coli Strains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise
James R. Johnson,Adam L. Stell +1 more
TL;DR: These findings provide novel insights into the VFs of extraintestinal pathogenic E. coli and demonstrate the new PCR assay's utility for molecular epidemiological studies.
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Global spread of antibiotic resistance: the example of New Delhi metallo-β-lactamase (NDM)- mediated carbapenem resistance
Alan P. Johnson,Neil Woodford +1 more
TL;DR: The spread of NDM illustrates that antibiotic resistance is a public health problem that transcends national borders and will require international cooperation between health authorities if it is to be controlled.