Characterizations of coronavirus cis-acting rna elements and the transcription step affecting its transcription efficiency
Sungwhan An,Shinji Makino +1 more
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The relative molar ratios of coronavirus m RNAs seem to have been established prior to a putative replicon-type amplification of subgenomic mRNAs.Citations
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Book ChapterDOI
The molecular biology of coronaviruses.
TL;DR: This review summarizes both classical and contemporary discoveries in the study of the molecular biology of these infectious agents, with particular emphasis on the nature and recognition of viral receptors, viral RNA synthesis, and the molecular interactions governing virion assembly.
Journal ArticleDOI
Nidovirus transcription: how to make sense…?
TL;DR: Nidovirus transcription mechanisms are compared, the experimental systems used are critically assessed and, in particular, the impact of recently developed reverse genetic systems is discussed.
Journal ArticleDOI
Subgenomic messenger RNA amplification in coronaviruses.
Hung-Yi Wu,David A. Brian +1 more
TL;DR: After transfection of an in vitro–generated marked sgmRNA into virus-infected cells, the sgm RNA can function as a template for (−)-strand synthesis, and in principle, the longer-nested sgmRNAs in a natural infection, all of which contain potential internal template-switching donor signals, can function to increase the number of the shorter 3′-nesting sgm RNAs.
Journal ArticleDOI
Engineering the Transmissible Gastroenteritis Virus Genome as an Expression Vector Inducing Lactogenic Immunity
TL;DR: The engineered vector led to expression levels as high as those of well-established vectors and seems very promising for the development of vaccines and, possibly, for gene therapy.
Journal ArticleDOI
Characterization of an Equine Arteritis Virus Replicase Mutant Defective in Subgenomic mRNA Synthesis
TL;DR: This study showed that EAV030F still produces subgenomic positive and negative strands, albeit at a very low level, suggesting that nsp10 plays an important role in an early step of EAV mRNA transcription.
References
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Journal ArticleDOI
An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.
Journal ArticleDOI
Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis.
TL;DR: Findings in 17CL1 mouse cells that had been infected with the A59 strain of mouse hepatitis virus strongly suggest that coronaviruses utilize a novel replication strategy that employs the synthesis of subgenome negative strands to produce subgenomic mRNAs.
Journal ArticleDOI
Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons
TL;DR: It is proposed that subgenomic mRNA replication, in addition to leader-primed transcription, is a significant mechanism of mRNA synthesis and that it functions to amplify mRNAs.
Journal ArticleDOI
Coronavirus mRNA synthesis involves fusion of non-contiguous sequences.
Willy J. M. Spaan,H. Delius,Michael A. Skinner,John D. Armstrong,Peter J. M. Rottier,Sjef Smeekens,B. A. M. Van Der Zeijst,Stuart G. Siddell +7 more
TL;DR: Positive‐stranded genomic RNA of coronavirus MHV and its six subgenomic mRNAs are synthesized in the cytoplasm of the host cell by a mechanism which appears to involve an unusual and specific ‘polymerase jumping’ event.