Journal ArticleDOI
Chemical characterization of silicon-substituted hydroxyapatite.
TLDR
Chemical analysis confirmed the proposed substitution of the silicon (or silicate) ion for the phosphorus (or phosphate) ion in hydroxyapatite and demonstrated that phase-pure silicon-substituted hydroxyAPatite may be prepared using a simple precipitation technique.Abstract:
Bioceramic specimens have been prepared by incorporating a small amount of silicon (0.4 wt %) into the structure of hydroxyapatite [Ca10(PO4)6(OH)2, HA] via an aqueous precipitation reaction to produce a silicon-substituted hydroxyapatite (Si-HA). The results of chemical analysis confirmed the proposed substitution of the silicon (or silicate) ion for the phosphorus (or phosphate) ion in hydroxyapatite. The Si-HA was produced by first preparing a silicon-substituted apatite (Si-Ap) by a precipitation process. A single-phase Si-HA was obtained by heating/calcining the as-prepared Si-Ap to temperatures above 700 degrees C; no secondary phases, such as tricalcium phosphate (TCP), tetracalcium phosphate (TeCP), or calcium oxide (CaO), were observed by X-ray diffraction analysis. Although the X-ray diffraction patterns of Si-HA and stoichiometric HA appeared to be identical, refinement of the diffraction data revealed some small structural differences between the two materials. The silicon substitution in the HA lattice resulted in a small decrease in the a axis and an increase in the c axis of the unit cell. This substitution also caused a decrease in the number of hydroxyl (OH) groups in the unit cell, which was expected from the proposed substitution mechanism. The incorporation of silicon in the HA lattice resulted in an increase in the distortion of the PO4 tetrahedra, indicated by an increase in the distortion index. Analysis of the Si-HA by Fourier transform infrared (FTIR) spectroscopy indicated that although the amount of silicon incorporated into the HA lattice was small, silicon substitution appeared to affect the FTIR spectra of HA, in particular the P-O vibrational bands. The results demonstrate that phase-pure silicon-substituted hydroxyapatite may be prepared using a simple precipitation technique.read more
Citations
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Journal ArticleDOI
Preparation and Characterization of Nanostructured Hydroxyapatite Using a Biomaterial
TL;DR: In this paper, a simple approach using biomaterial (egg shell membrane) and characterized by Fourier transform infra-red spectroscopy (FT-IR), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscope (TEM) techniques was presented.
Journal ArticleDOI
In Vitro Analysis of Protein Adhesion to Phase Pure Hydroxyapatite and Silicon Substituted Hydroxyapatite
Cláudia Botelho,Roger A. Brooks,Takahiro Kawai,Shinichi Ogata,Chikara Ohtsuki,Serena M. Best,Lopes,José D. Santos,Neil Rushton,William Bonfield +9 more
TL;DR: The results suggest that the increased bioactivity seen with Si-HA is not due to the degree of protein adhesion, but may possibly be due to changes in the conformation of the bound proteins.
Book ChapterDOI
Design and Development of Ceramics and Glasses
TL;DR: A synergistic combination of bioactive materials and cell therapy offers an attractive approach to promote tissue regeneration; such smart biofunctionalization strategies will continue to play a key role in tissue engineering and regenerative medicine.
Journal ArticleDOI
Biological and Physical-Chemical Characterization of Phase Pure HA and SI-Substituted Hydroxyapatite by Different Microscopy Techniques
Cláudia Botelho,Roger A. Brooks,Serena M. Best,Lopes,José D. Santos,Neil Rushton,William Bonfield +6 more
TL;DR: In this article, two different microscopy techniques were used to investigate the re sponse of human osteoblasts to hydroxyapatite (HA) and silicon substituted Hydroxyapati te (Si-HA), namely, fluorescence and confocal microscopy, after incubation for different periods of time in simulated body fluid, were ased using atomic force microscopy and environmental electron scanning microscopy.
Journal ArticleDOI
Characterization of silicon-substituted hydroxyapatite powders synthesized by a wet precipitation method
TL;DR: In this article, the effects of the Si substitution on crystallite size, lattice parameters and fraction of crystalline phase of the powders were discussed using a wet precipitation method.
References
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TL;DR: The mechanisms of tissue bonding to bioactive ceramics are beginning to be understood, which can result in the molecular design of bioceramics for interfacial bonding with hard and soft tissues.
Journal Article
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