Journal ArticleDOI
Chemotactic activity in inflammatory bowel disease. Role of leukotriene B4
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TLDR
Data suggest that leukotriene B4 is an important stimulus to neutrophil chemotaxis in inflammatory bowel disease and, thus, may play a major role in the amplification of the inflammatory response in this condition.Abstract:
An important histologic feature of inflammatory bowel disease (IBD) is infiltration of the colonic mucosa with neutrophils. To investigate the nature of the chemotactic agents responsible for this infiltration, colonic mucosa from three normals and nine patients with inflammatory bowel disease (seven ulcerative colitis, two Crohn's colitis) was assayed for chemotactic activity for human neutrophils in vitro in a Boyden chamber. There was more (greater than 10-fold more) chemotactic activity in homogenates of inflammatory bowel disease mucosa than in homogenates of normal colonic mucosa. Analysis of the chemotactic activity in the inflammatory bowel disease mucosa revealed that most was lipid extractable. Moreover, when the lipid extract was fractionated by reverse-phase high-pressure liquid chromatography, the only fraction with significant chemotactic activity was the fraction that coeluted with leukotriene B4. The chemotactic response to IBD mucosa was blocked by anti-LTB4 antisera. The amount of chemotactic activity in lipid extracts of different inflammatory bowel disease specimens correlated well with the concentration of leukotriene B4 measured by UV absorbance (250 ng/g of mucosa). These data suggest that leukotriene B4 is an important stimulus to neutrophil chemotaxis in inflammatory bowel disease and, thus, may play a major role in the amplification of the inflammatory response in this condition.read more
Citations
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Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip
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Eicosanoids and the gastrointestinal tract
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Dietary supplementation with fish oil in ulcerative colitis.
William F. Stenson,David Cort,John Rodgers,Robert Burakoff,Katherine DeSchryver-Kecskemeti,Terri L. Gramlich,Warren Beeken +6 more
TL;DR: Four months of diet supplementation with fish oil in patients with inflammatory bowel disease resulted in reductions in rectal dialysate leukotriene B4 levels, improvements in histologic findings, and weight gain.
Journal ArticleDOI
Treatment of ulcerative colitis with fish oil supplementation: a prospective 12 month randomised controlled trial.
A. B. Hawthorne,T. K. Daneshmend,Christopher J. Hawkey,Andrea Belluzzi,S. J. Everitt,G. K. T. Holmes,C. Malkinson,M. Z. Shaheen,J. E. Willars +8 more
TL;DR: It is concluded that fish oil supplementation produces a modest corticosteroid sparing effect in active disease, but there is no benefit in maintenance therapy.
References
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Journal ArticleDOI
Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes
TL;DR: The chemokinetic and aggregating activities released from rat and human PMNs exposed to ionophore A23187 are not due to the release of mono-HETEs but to that of 5,12-di- HETE (leukotriene B), which is active over the concentration range 10 pg ml−1 to 5 ng ml-1.
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Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models
TL;DR: In this paper, an assay was devised to quantitate acute intestinal inflammation based on the assessment of myeloperoxidase activity, an enzyme found in neutrophils and, in much smaller quantities, in monocytes and macrophages.
Journal ArticleDOI
Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: in vivo effects with relevance to the acute inflammatory response
Sven-Erik Dahlén,J Björk,Per Hedqvist,K E Arfors,Sven Hammarström,Jan Åke Lindgren,Bengt Samuelsson +6 more
TL;DR: It is suggested that leukotrienes, formed in several blood-borne and tissue-bound cells, may mediate important microcirculatory adjustments to noxious stimuli.