Chondrocytes cultured in silk-based biomaterials maintain function and cell morphology.
TLDR
Regenerated silk fibroin film was found to be an excellent biomaterial with good cytocompatibility for chondrocytes, because these cells remained functional and maintained normal cell morphology when cultured in silk-based biomaterials.Abstract:
Objective:To characterize the morphology of chondrocytes and the expression and secretion of active collagen II by these cells cultured within a regenerated silk fibroin film. Silk fibroin film cyt...read more
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Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon Stem/Progenitor Cells.
Kang Lu,Xiaodie Chen,Hong Tang,Mei Zhou,Gang He,Juan Liu,Bian Xuting,Guo Yupeng,Fan Lai,Yang Mingyu,Zhisong Lu,Kanglai Tang +11 more
TL;DR: SF film with a bionic microstructure can serve as a tissue engineering scaffold and provide biophysical cues for the use of TSPCs to achieve proper cellular adherence arrangement and morphology as well as promote the tenogenic differentiation of T SPCs, making it a valuable customizable biomaterial for future applications in tendon repair.
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Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase.
TL;DR: SF films with a bionic microstructure may serve as a scaffold, provide biophysical cues to alter the cellular adherence arrangement and cell morphology, and enhance the tenogenic gene and protein expression in TSPCs.
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A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
TL;DR: In this paper , a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA), which does not require any complicated instruments and can be visually colored, is presented.
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Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the α2β1/FAK/PI3K/AKT Signaling Pathway In Vitro
Kang Lu,Hong Tang,Yang Wang,Liyuan Wang,Mei Zhou,Gang He,Hao Lu,Chu Yue Tang,Wan Chen,Kanglai Tang,Zhong-Liang Deng +10 more
TL;DR: In this article , the authors explored the molecular pathways underlying micropattern SF film-regulated TSPC propensity and their repairing effects to highlight the application value of micro-parameter SF films.
Journal ArticleDOI
Evaluation of new robust silk fibroin hydrogels for posterior scleral reinforcement in rabbits
Yule Xu,Qiaolin Chen,Zhengzhong Shao,Jiahong Wei,Xuyou Zhu,Ao Rong,Xin Chen,Yusu Ni,Yi-Wen Jiang +8 more
TL;DR: In this article , robust regenerated silk fibroin (RSF) hydrogels were used as potential grafts for posterior scleral reinforcement in animal experiments to evaluate their safety and biological reactions.
References
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Journal ArticleDOI
Silk-based biomaterials
Gregory H. Altman,Frank Diaz,Caroline M. Jakuba,Tara Calabro,Rebecca L. Horan,Jingsong Chen,Helen H. Lu,John C. Richmond,David L. Kaplan +8 more
TL;DR: Studies with well-defined silkworm silk fibers and films suggest that the core silk fibroin fibers exhibit comparable biocompatibility in vitro and in vivo with other commonly used biomaterials such as polylactic acid and collagen.
Journal ArticleDOI
Silk matrix for tissue engineered anterior cruciate ligaments
Gregory H. Altman,Rebecca L. Horan,Helen H. Lu,Jodie E. Moreau,Ivan Martin,John C. Richmond,David L. Kaplan +6 more
TL;DR: The results support the conclusion that properly prepared silkworm fiber matrices, aside from providing unique benefits in terms of mechanical properties as well as biocompatibility and slow degradability, can provide suitable biomaterial matrices for the support of adult stem cell differentiation toward ligament lineages.
Journal ArticleDOI
Autologous chondrocyte implantation versus matrix-induced autologous chondrocyte implantation for osteochondral defects of the knee: a prospective, randomised study.
W. Bartlett,John A. Skinner,C.R. Gooding,R. W. J. Carrington,Adrienne M. Flanagan,T. W. R. Briggs,George E. Bentley +6 more
TL;DR: The clinical, arthroscopic and histological outcomes are comparable for both ACI-C and MACI, and while MACI is technically attractive, further long-term studies are required before the technique is widely adopted.
Journal ArticleDOI
The inflammatory responses to silk films in vitro and in vivo
Lorenz Meinel,Sandra Hofmann,Sandra Hofmann,Vassilis Karageorgiou,Carl A. Kirker-Head,J. McCool,Gloria Gronowicz,Ludwig Zichner,Robert Langer,Gordana Vunjak-Novakovic,David L. Kaplan +10 more
TL;DR: Biocompatibility studies of silk films (with or without covalently bound RGD) that were seeded with bone-marrow derived mesenchymal stem cells and cultured in vitro with human MSC suggest that purified degradable silk is biocompatible and the in vitro cell culture model gave inflammatory responses that were comparable to those observed in vivo.
Journal ArticleDOI
In vitro degradation of silk fibroin.
Rebecca L. Horan,Kathryn Antle,Adam L. Collette,Yongzhong Wang,Jia Huang,Jodie E. Moreau,Vladimir Volloch,David L. Kaplan,Gregory H. Altman +8 more
TL;DR: In this paper, a model system of proteolytic degradation of silk fibroin yarns was created to create an in vitro model system for degradable biomaterials which can slowly and predictably transfer a loadbearing burden to developing biological tissue.