Circulation of imipenem-resistant Acinetobacter baumannii ST10, ST2 and ST3 in a university teaching hospital from Tehran, Iran.
TLDR
A new two-locus typing method (blaOXA-51-like and ampC) method for the rapid identification of clonal complexes (CCs) that has more discrimination than the application of each method separately and it could be applied forThe rapid determination of the CC without performing MLST.Abstract:
Purpose Multi-drug resistant (MDR) Acinetobacter baumannii has introduced a worldwide health crisis. The purposes of this study were to characterize the clonal relatedness among MDR clinical strains and to introduce a new two-locus typing method confirmed by multi-locus sequence typing (MLST). Methodology In this study, we determined antimicrobial resistance, detected genes associated with carbapenem resistance and characterized clonal relatedness among 99 clinical isolates extracted from 82 hospitalized inpatients in a university hospital. Results Of the 99 A. baumannii isolates, 92.9% (92/99) were resistant to imipenem and 97.9% (97/99) had an MDR profile. We found that the high prevalence of blaVIM [94.9% (94/99)] and blaOXA-23-like [93.93% (93/99)] is the main mechanism of carbapenem resistance. This study proposes a new two-locus typing (blaOXA-51-like and ampC) method for the rapid identification of clonal complexes (CCs). The results of this method and confirmation by MLST show that clinical isolates carry blaOXA-68 as well as ampC-10 or ampC-20 genes belonging to CC10 (ST10); blaOXA-66 and ampC-2 belonging to CC2 (ST2); and blaOXA-71 and ampC-3 belonging to CC3 (ST3). One isolate had blaOXA-90 with an undetermined allele number of ampC belonging to ST513. Conclusion The high prevalence of MDR strains and the circulation of four limited clones, including ST10 (45/99), ST2 (41/99), ST3 (12/99) and ST513 (1/99), in the clinical setting highlights the importance of a rigorous infection control programme. The two-locus typing method has more discrimination than the application of each method separately and it could be applied for the rapid determination of the CC without performing MLST.read more
Citations
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The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals.
Rahimeh Sanikhani,Mohammad Moeinirad,Hamid Solgi,Azar Hadadi,Fereshteh Shahcheraghi,Farzad Badmasti +5 more
TL;DR: It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to CG23 and high resistance to imipenem indicated that there is an urgent problem that should be addressed in the clinical settings.
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Isolation of extensively drug resistant Acinetobacter baumannii from environmental surfaces inside intensive care units
TL;DR: A. baumannii is a nosocomial pathogen that has emerged as a major threat in the health-care settings, particularly intensive care units (ICUs), particularly ICUs.
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Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients.
TL;DR: In this article, the authors performed a cross-sectional study on 106 non-duplicate Acinetobacter baumannii isolates from burn patients referred to Shahid Motahari Hospital in Tehran.
Journal ArticleDOI
Isolation of extensively drug resistant Acinetobacter baumannii from environmental surfaces inside intensive care units
TL;DR: A. baumannii is a nosocomial pathogen that has emerged as a major threat in the health-care settings, particularly intensive care units (ICUs), particularly ICUs as mentioned in this paper .
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Acquired mucoid phenotype of Acinetobacter baumannii: Impact for the molecular characteristics and virulence.
Wulin Shan,Huanhuan Zhang,Jinsong Kan,Meiling Yin,Jiayun Zhang,Lingling Wan,Renliang Chang,Ming Li +7 more
TL;DR: In this paper, the impact of the mucoid phenotype and antibiotic resistance on the virulence of Acinetobacter baumannii was analyzed, and the authors provided novel insights for the prevention and treatment of infections associated with these strains.
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