Comparative genomic analysis of the family Iridoviridae : re-annotating and defining the core set of iridovirus genes
TLDR
The re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses, and further re-defining the core set of iridovirus genes will continue to lead to a better understanding of the phylogenetic relationships between individual irids.Abstract:
Members of the family Iridoviridae can cause severe diseases resulting in significant economic and environmental losses. Very little is known about how iridoviruses cause disease in their host. In the present study, we describe the re-analysis of the Iridoviridae family of complex DNA viruses using a variety of comparative genomic tools to yield a greater consensus among the annotated sequences of its members. A series of genomic sequence comparisons were made among, and between the Ranavirus and Megalocytivirus genera in order to identify novel conserved ORFs. Of these two genera, the Megalocytivirus genomes required the greatest number of altered annotations. Prior to our re-analysis, the Megalocytivirus species orange-spotted grouper iridovirus and rock bream iridovirus shared 99% sequence identity, but only 82 out of 118 potential ORFs were annotated; in contrast, we predict that these species share an identical complement of genes. These annotation changes allowed the redefinition of the group of core genes shared by all iridoviruses. Seven new core genes were identified, bringing the total number to 26. Our re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses. Further re-defining the core set of iridovirus genes will continue to lead us to a better understanding of the phylogenetic relationships between individual iridoviruses as well as giving us a much deeper understanding of iridovirus replication. In addition, this analysis will provide a better framework for characterizing and annotating currently unclassified iridoviruses.read more
Citations
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Journal ArticleDOI
Discovery of Novel Viruses Associated With the Invasive Cane Toad (Rhinella marina) in Its Native and Introduced Ranges.
Alice G. Russo,Emma F Harding,Grace J. H. Yan,Daniel Selechnik,Daniel Selechnik,Simon Ducatez,Jayna L. DeVore,Jia Zhou,Roshmi R. Sarma,Yin Peng Lee,Mark F. Richardson,Richard Shine,Lee A. Rollins,Lee A. Rollins,Peter A. White +14 more
TL;DR: The authors compared the prevalence and diversity between cane toads in their native range (French Guiana, n=25) and two introduced ranges (Hawai'i and Australia) with a metatranscriptomic and metagenomic approach combined with PCR screening.
Journal ArticleDOI
Histopathological evaluation and molecular detection of natural Iridovirus infection in cultured grouper fish in Malaysia
M . Hazeri,M. D. Hassan,Yusuf Abba,Abdul Rahman Omar,Z. N. Allaudin,Mehdi Soltani,Ruhil Hayati Hamdan,N. F. A. Mohamad,S. M. Raina,M. S. Vishkaei +9 more
TL;DR: Based on the gross, histopathological and molecular detection GIV infection was established in naturally infected grouper farms in Malaysia, and basophilic or eosinophilic enlarged cells with the presence of mononuclear cellular infiltrations were seen in the kidney, liver, eye and gills, which is the distinctive feature of this disease.
Book ChapterDOI
Antiviral Immunity in the Fruit Fly, Drosophila melanogaster
TL;DR: The fruit fly, Drosophila melanogaster, is an extremely useful model to study innate immunity mechanisms, and at least three pathways show signal integration in response to viral infection, demonstrating a coordinated immune response against viral infection.
Journal ArticleDOI
Singapore Grouper Iridovirus ORF75R is a Scaffold Protein Essential for Viral Assembly
TL;DR: The data suggest that ORF75R is a novel scaffold protein important for viral assembly and DNA encapsidation, but its phosphorylation facilitates virion disassembly.
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Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions.
Yesim Akturk Dizman,Yesim Akturk Dizman,Hacer Muratoglu,Cemal Sandalli,Remziye Nalcacioglu,Zihni Demirbag +5 more
TL;DR: Biochemical characterization of the purified CIV 012L confirmed that this viral protein is a functional 5′-3′ exonuclease that digests 3′-biotin-labelled oligonucleotides and linear double-stranded DNA (dsDNA) molecules from their 5′ termini in a highly processive manner.
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