Comparative genomic analysis of the family Iridoviridae : re-annotating and defining the core set of iridovirus genes
TLDR
The re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses, and further re-defining the core set of iridovirus genes will continue to lead to a better understanding of the phylogenetic relationships between individual irids.Abstract:
Members of the family Iridoviridae can cause severe diseases resulting in significant economic and environmental losses. Very little is known about how iridoviruses cause disease in their host. In the present study, we describe the re-analysis of the Iridoviridae family of complex DNA viruses using a variety of comparative genomic tools to yield a greater consensus among the annotated sequences of its members. A series of genomic sequence comparisons were made among, and between the Ranavirus and Megalocytivirus genera in order to identify novel conserved ORFs. Of these two genera, the Megalocytivirus genomes required the greatest number of altered annotations. Prior to our re-analysis, the Megalocytivirus species orange-spotted grouper iridovirus and rock bream iridovirus shared 99% sequence identity, but only 82 out of 118 potential ORFs were annotated; in contrast, we predict that these species share an identical complement of genes. These annotation changes allowed the redefinition of the group of core genes shared by all iridoviruses. Seven new core genes were identified, bringing the total number to 26. Our re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses. Further re-defining the core set of iridovirus genes will continue to lead us to a better understanding of the phylogenetic relationships between individual iridoviruses as well as giving us a much deeper understanding of iridovirus replication. In addition, this analysis will provide a better framework for characterizing and annotating currently unclassified iridoviruses.read more
Citations
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Journal ArticleDOI
Genomic Sequencing of Ranaviruses Isolated from Turbot (Scophthalmus maximus) and Atlantic Cod (Gadus morhua).
Ellen Ariel,Natalie K. Steckler,Kuttichantran Subramaniam,Niels Jørgen Olesen,Thomas B. Waltzek +4 more
TL;DR: Phylogenomic analyses revealed that these two ranaviruses are nearly identical and form a distinct clade at the base of the ranavirus tree branching off near other fish ranavIRuses.
Dissertation
Emergence of a virulent wildlife disease: using spatial epidemiology and phylogenetic methods to reconstruct the spread of amphibian viruses
TL;DR: Improved characterization of the disease process associated with ranavirus infection would enable a priori filtering of ranvirus-consistent reports and provision of better guidance to citizen-scientists about when, where and how to carry out surveillance.
Journal ArticleDOI
Rana grylio virus TK and DUT gene locus could be simultaneously used for foreign gene expression
TL;DR: A novel dual-fluorescence labeled recombinant iridovirus in which DUT and TK gene locus were simultaneously used for foreign gene expression was constructed.
Journal ArticleDOI
Isolation, identification and genomic analysis of an ISKNV-type megalocytivirus from spotted knifejaw (Oplegnathus punctatus)
Xiaohong Huang,Jingguang Wei,Qi Zheng,Ya Zhang,Weibin Zhu,Jiangchun Liu,Yunxia Hou,Qiwei Qin,Youhua Huang +8 more
TL;DR: It is speculated that SKIV-SD, an ISKNV-type megalocytivirus, was a lethal viral pathogen to cultured spotted knifejaw.
Universidade de são paulo faculdade de zootecnia e engenharia de alimentos
TL;DR: The results suggest that the presence of TAMs and eosinophils in cutaneous canine MCTs are not good prognostic indicators for this disease.
References
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