Comparative genomic analysis of the family Iridoviridae : re-annotating and defining the core set of iridovirus genes
TLDR
The re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses, and further re-defining the core set of iridovirus genes will continue to lead to a better understanding of the phylogenetic relationships between individual irids.Abstract:
Members of the family Iridoviridae can cause severe diseases resulting in significant economic and environmental losses. Very little is known about how iridoviruses cause disease in their host. In the present study, we describe the re-analysis of the Iridoviridae family of complex DNA viruses using a variety of comparative genomic tools to yield a greater consensus among the annotated sequences of its members. A series of genomic sequence comparisons were made among, and between the Ranavirus and Megalocytivirus genera in order to identify novel conserved ORFs. Of these two genera, the Megalocytivirus genomes required the greatest number of altered annotations. Prior to our re-analysis, the Megalocytivirus species orange-spotted grouper iridovirus and rock bream iridovirus shared 99% sequence identity, but only 82 out of 118 potential ORFs were annotated; in contrast, we predict that these species share an identical complement of genes. These annotation changes allowed the redefinition of the group of core genes shared by all iridoviruses. Seven new core genes were identified, bringing the total number to 26. Our re-analysis of genomes within the Iridoviridae family provides a unifying framework to understand the biology of these viruses. Further re-defining the core set of iridovirus genes will continue to lead us to a better understanding of the phylogenetic relationships between individual iridoviruses as well as giving us a much deeper understanding of iridovirus replication. In addition, this analysis will provide a better framework for characterizing and annotating currently unclassified iridoviruses.read more
Citations
More filters
Journal ArticleDOI
Genotype determination of megalocytivirus from Indonesian Marine Fishes
TL;DR: This paper is the first report on the present of ISKNV and RSIV genotypes in Indonesia based on MCP, ATPase, DNA polymerase, CY15 and IRB6 genes.
Book ChapterDOI
Iridoviruses of Vertebrates
A.D. Hyatt,V.G. Chinchar +1 more
TL;DR: All groups have the potential to cause morbidity and mortality in their respective hosts with representatives of all, except the erthrocytic necrosis viruses, being recognized by the Office International des Epizooties (OIE) as causing diseases of importance in species involved in international trade.
Biology of a small RNA virus that infects Drosophila melanogaster
TL;DR: Drosophila melanogaster has been extensively used as a model organism to study diverse facets of biology, including host-pathogen interactions and the basic biology of its pathogens.
Journal ArticleDOI
DNAJA4 Promotes the Replication of the Chinese Giant Salamander Iridovirus
TL;DR: The role of DNAJA4 (DnaJ Heat Shock Protein Family (Hsp40) Member A4) in the ranavirus infection has not been reported as discussed by the authors .
Ranaviruses: Detection, differentiation and host immune response
TL;DR: The results indicate that persistent ranavirus infections may occur in certain conditions and that rainbow trout could act as a vector species for ranaviruses.
References
More filters
Journal ArticleDOI
Emerging infectious diseases and amphibian population declines.
TL;DR: The role of these diseases in the global decline of amphibian populations is examined and hypotheses for the origins and impact of these panzootics are proposed.
Journal ArticleDOI
Infectious disease and amphibian population declines
TL;DR: It is suggested that, in common with many emerging infectious diseases of humans, domestic animals and other wildlife species, emergence of chytridiomycosis may be driven by anthropogenic introduction (pathogen pollution).
Journal ArticleDOI
Measuring genome evolution.
Martijn A. Huynen,Peer Bork +1 more
TL;DR: A hierarchy of rates at which genomes have changed during evolution is established and it is shown that some genomes are more highly organized than others: they show a higher degree of the clustering of genes that have orthologs in other genomes.
Journal ArticleDOI
Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus.
TL;DR: It is suggested that ISKNV, RSIV, sea bass iridovirus, grouper iridvirus, and African lampeye iridavirus may belong to a new genus of the Iridoviridae family and are tentatively referred to as cell hypertrophy iridOViruses.