Journal ArticleDOI
Comparison of anaerobic dechlorinating enrichment cultures maintained on tetrachloroethene, trichloroethene, cis-dichloroethene and vinyl chloride
Melanie Duhamel,Stephan D. Wehr,Lawrence Yu,Homa Rizvi,D. J. Seepersad,Sandra Dworatzek,E. Cox,Elizabeth A. Edwards +7 more
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TLDR
Differences in rates and community composition developed between the different subcultures, including the loss of the VC enrichment culture's ability to dechlorinate PCE, and it is apparent that significant mechanistic differences exist between each step of dechlorination from TCE to ethene, especially for the last important de chlorination step from VC to e thene.About:
This article is published in Water Research.The article was published on 2002-10-01. It has received 287 citations till now. The article focuses on the topics: Dehalococcoides & Enrichment culture.read more
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Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium.
TL;DR: An unusual, strictly anaerobic bacterium is described that destroys dichloroethenes and vinyl chloride as part of its energy metabolism, generating environmentally benign products (biomass, ethene and inorganic chloride).
Journal ArticleDOI
Field demonstration of successful bioaugmentation to achieve dechlorination of tetrachloroethene to ethene.
David W. Major,Michaye L. McMaster,E. Cox,Elizabeth A. Edwards,Sandra Dworatzek,Edwin R. Hendrickson,Mark G. Starr,Jo Ann Payne,Lois W. Buonamici +8 more
TL;DR: Biostimulation and bioaugmentation to dechlorinate tetrachloroethene (PCE) to ethene at Kelly Air Force Base confirmed that organisms in the KB-1 culture populated the PTA aquifer and contributed to the stimulation of dechlorination beyond cDCE to e thene.
Journal ArticleDOI
Anaerobic microbial dehalogenation.
Hauke Smidt,Willem M. de Vos +1 more
TL;DR: This contribution provides an update on the current knowledge on metabolic and phylogenetic diversity of anaerobic microorganisms that are capable of dehalogenating--or completely mineralizing--halogenated hydrocarbons by fermentative, oxidative, or reductive pathways.
Journal ArticleDOI
Complete detoxification of vinyl chloride by an anaerobic enrichment culture and identification of the reductively dechlorinating population as a Dehalococcoides species.
TL;DR: It is demonstrated that Dehalococcoides populations exist that use DCEs and VC but not PCE or TCE as metabolic electron acceptors, and 16S rRNA gene-based tools identified and quantitative real-time PCR confirmed VC-dependent growth of this population.
Journal ArticleDOI
Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe.
TL;DR: A strictly anaerobic bacterium was isolated from tetrachloroethene (PCE)-to-ethene dechlorinating microcosms established with river sediment without prior exposure to chlorinated solvents, and it was suggested that strain FL2 captures energy from both the TCE-to-cis-DCE and 1,2-D CE- to-VC dechlorination steps.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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TL;DR: Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment.
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The rdp (ribosomal database project)
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TL;DR: The Ribosomal Database Project (RDP-II), previously described by Maidak et al. (2000), continued during the past year to add new rRNA sequences to the aligned data and to improve the analysis commands.
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Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene
TL;DR: Growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did not react with a peptidoglycan-specific lectin and its ultrastructure resembled S-layers of Archaea.