Journal ArticleDOI
Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene
TLDR
Growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did not react with a peptidoglycan-specific lectin and its ultrastructure resembled S-layers of Archaea.Abstract:
Tetrachloroethene is a prominent groundwater pollutant that can be reductively dechlorinated by mixed anaerobic microbial populations to the nontoxic product ethene. Strain 195, a coccoid bacterium that dechlorinates tetrachloroethene to ethene, was isolated and characterized. Growth of strain 195 with H2 and tetrachloroethene as the electron donor and acceptor pair required extracts from mixed microbial cultures. Growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did not react with a peptidoglycan-specific lectin and its ultrastructure resembled S-layers of Archaea. Analysis of the 16S ribosomal DNA sequence of strain 195 indicated that it is a eubacterium without close affiliation to any known groups.read more
Citations
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Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity
TL;DR: The authors' perspective on microbial diversity has improved enormously over the past few decades in large part due to molecular phylogenetic studies that objectively relate organisms.
Journal Article
Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity (vol 180, pg 4765, 1998)
Journal ArticleDOI
The Uncultured Microbial Majority
TL;DR: Genome sequence information that would allow ribosomal RNA gene trees to be related to broader patterns in microbial genome evolution is scant, and therefore microbial diversity remains largely unexplored territory.
Journal ArticleDOI
Occurrence, classification, and biological function of hydrogenases: an overview.
Journal ArticleDOI
Novel division level bacterial diversity in a Yellowstone hot spring.
TL;DR: A culture-independent molecular phylogenetic survey was carried out for the bacterial community in Obsidian Pool (OP), a Yellowstone National Park hot spring previously shown to contain remarkable archaeal diversity.
References
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Journal ArticleDOI
The Ribosomal Database project
Niels Larsen,Gary J. Olsen,Bonnie L. Maidak,Michael J. McCaughey,Ross Overbeek,Thomas J. Macke,Terry L. Marsh,Carl R. Woese +7 more
TL;DR: The Ribosomal Database Project (RDP) is a curated database that offers ribosome data along with related programs and services that include phylogenetically ordered alignments of ribosomal RNA (rRNA) sequences, derived phylogenetic trees, rRNA secondary structure diagrams and various software packages for handling, analyzing and displaying alignments and trees.
Journal ArticleDOI
The Phylogeny of the Genus Clostridium: Proposal of Five New Genera and Eleven New Species Combinations
Matthew D. Collins,Paul A. Lawson,Anne Willems,Juan J. Córdoba,José F. Fernández-Garayzábal,P. Garcia,J. Cai,Hans Hippe,John A. E. Farrow +8 more
TL;DR: The 16S rRNA gene sequences were determined by PCR direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- G + C-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of theClostridia.
Journal ArticleDOI
Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions.
TL;DR: Studies with enrichment cultures of PCE- and TCE-degrading microorganisms provide evidence that, under methanogenic conditions, mixed cultures are able to completely dechlorinate PCE and T CE to ethylene, a product which is environmentally acceptable.
Journal ArticleDOI
Microbial reductive dehalogenation.
William W. Mohn,James M. Tiedje +1 more
TL;DR: Agarwal et al. as mentioned in this paper used Desulfomonile tiedjei DCB-1 as a model to understand reductive dehalogenating organisms in undefined, syntrophic anaerobic communities.
Journal ArticleDOI
A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth
TL;DR: H2 consumption was paralleled by chloride production, PCE degradation, cis-1,2-DCE formation, and growth of PER-K23, and Electron balances showed that all electrons derived from H2 or formate consumption were recovered in dechlorination products and biomass.
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