Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates.
S M Rodger,I H Holmes +1 more
TLDR
By co-electrophoresis in polyacrylamide gels, the segmented double-standed RNA genome of the simian rotavirus, SA 11, was compared with those of human and bovine rotaviruses and a scheme for the systematic designation of different rotav virus samples is proposed.Abstract:
By co-electrophoresis in polyacrylamide gels, the segmented double-standed RNA genome of the simian rotavirus, SA 11, was compared with those of human and bovine rotaviruses. A comparison between SA 11 virus and the Northern Ireland cell culture adapted bovine virus showed that the electrophoretic mobilities of each of the 11 corresponding segments differed. In other comparisons, four to seven segment variations were more common. When the genomes of various bovine rotaviruses were compared, eight different electropherotypes were detected. Four of these electropherotypes were obtained from one property during a single outbreak of disease. In view of such genetic diversity, a scheme for the systematic designation of different rotavirus samples is proposed. The significance of the variations in relation to the molecular epidemiology of bovine rotavirus infections is discussed.read more
Citations
More filters
Journal ArticleDOI
Rotavirus gene structure and function.
Mary K. Estes,Jean Cohen +1 more
TL;DR: Detailed analyses with recently characterized immunologic and gene probes and new animal models can be expected to provide a basic understanding of what regulates the primary interactions of these viruses with the gastrointestinal tract and the subsequent responses of infected hosts.
Book ChapterDOI
Rotaviruses: A Review
TL;DR: The Rotavirus genus of the Reoviridae consists of a number of antigenically related and morphologically identical viruses that cause enteritis, primarily in young mammals and avians.
Journal ArticleDOI
Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid.
TL;DR: Electrophoretic typing techniques can be applied routinely and reproducibly to small samples of feces and could prove to be of value in epidemiological studies of rotavirus infection.
Journal ArticleDOI
Molecular Characterization of Rotaviruses with Distinct Group Antigens
TL;DR: Experimental criteria have been established which allow the division of rotaviruses into three groups and it is indicated that the viruses in the three groups did not carry any genome segments in common with each other.
Journal ArticleDOI
Nucleotide sequence heterogeneity of the RNA from a natural population of foot-and-mouth-disease virus.
TL;DR: Oligonucleotide mapping indicates that the variant oligonucleotides are scattered throughout the FMDV genome, suggesting that extensive genetic variability at many RNA sites is the basis for the antigenic diversity of F MDV.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
Journal ArticleDOI
Hybridization and sedimentation studies on “early” and “late” vaccinia messenger RNA
TL;DR: The pattern of macromolecular biosynthesis during the vaccinia virus multiplication cycle, that is, early and late messenger RNA transcribed, has been studied by means of hybridization and density gradient sedimentation.
Journal ArticleDOI
Differences in RNA patterns of influenza A viruses.
Peter Palese,Jerome L. Schulman +1 more
TL;DR: Analysis of the segmented RNAs of influenza A viruses by electrophoresis on polyacrylamide urea slab gels has provided a method for sharper resolution of the number and migration rates of different segments, and may be possible to define which RNA segment codes for each viral protein.