Compilation and analysis of Escherichia coli promoter DNA sequences.
TLDR
A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations.Abstract:
The DNA sequence of 168 promoter regions (-50 to +10) for Escherichia coli RNA polymerase were compiled. The complete listing was divided into two groups depending upon whether or not the promoter had been defined by genetic (promoter mutations) or biochemical (5' end determination) criteria. A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations. In addition, we have tabulated 98 promoter mutations. Nearly all of the altered base pairs in the mutants conform to the following general rule: down-mutations decrease homology and up-mutations increase homology to the consensus sequence.read more
Citations
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Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
Craig Tuerk,Larry Gold +1 more
TL;DR: High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species.
Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
Journal ArticleDOI
Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product.
TL;DR: Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid, and the Iap protein seems to be associated with the membrane.
Journal ArticleDOI
DNA binding sites: representation and discovery.
TL;DR: The purpose of this article is to provide a brief history of the development and application of computer algorithms for the analysis and prediction of DNA binding sites.
Journal ArticleDOI
Vectors for selective expression of cloned DNAs by T7 RNA polymerase
TL;DR: Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribing by T7 RNA polymerases, in vitro or in E. coli cells.
References
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Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription
Martin Rosenberg,Donald L. Court +1 more
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.
Journal ArticleDOI
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
TL;DR: Comparison of the sequence of λrifd18 with sequences from other isolates of the rrB operon provides direct evidence for structural rearrangements within rRNA operons.
Journal ArticleDOI
Nucleotide sequence of the rightward operator of phage lambda
TL;DR: The sequence of 72 base pairs of the rightward operator (O-R) of bacteriophage lambda is presented as determined with simple and rapid methods for direct DNA sequencing and three sites recognized by the lambda phage repressor are identified.