Complete amino acid sequence of a new type of lethal neurotoxin from the venom of the funnel-web spider Atrax robustus.
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TLDR
The disposition of disulphide–bridged cysteine residues at both the amino– and carboxy–termini and as a triplet at residues 14–16 appears to have no precedent amongst neurotoxins.About:
This article is published in FEBS Letters.The article was published on 1985-02-11 and is currently open access. It has received 80 citations till now. The article focuses on the topics: Atrax & Venom.read more
Citations
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The cystine knot structure of ion channel toxins and related polypeptides
TL;DR: The compact and robust nature of this motif makes it an excellent scaffold for the design and engineering of novel polypeptides with enhanced activity against existing targets, or with activity against novel targets.
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A rational nomenclature for naming peptide toxins from spiders and other venomous animals.
TL;DR: A rational nomenclature is introduced that can be applied to the naming of peptide toxins from spiders and other venomous animals to enable these toxins to be rationally classified, catalogued, and compared.
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Structure and pharmacology of spider venom neurotoxins
TL;DR: Following diverse molecular evolution mechanisms, and in particular selective hypermutation, short spider peptides appear to have functionally diversified while retaining a conserved molecular scaffold.
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Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta.
W S Skinner,Michael E. Adams,Gary B. Quistad,Hiroshi Kataoka,B J Cesarin,F. E. Enderlin,David A. Schooley +6 more
TL;DR: Two classes of paralytic toxins were isolated from the venom of Agelenopsis aperta and their chemical and larvicidal properties characterized and the secondary structure of one of these toxins (mu-Aga V) was investigated.
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Venom landscapes: Mining the complexity of spider venoms via a combined cDNA and mass spectrometric approach
TL;DR: This work shows that the venoms of Australian funnel-web spiders contain many hundreds of peptides that follow a bimodal distribution, and proposes that three-dimensional 'venom landscapes' derived from LC-MALDI analysis might have predictive value for the discovery of various groups of pharmacologically distinct toxins in complex venoms.
References
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A gas-liquid solid phase peptide and protein sequenator.
TL;DR: A new miniaturized protein and peptide sequenator has been constructed which uses gas phase reagents at the coupling and cleavage steps of the Edman degradation, characterized by a high repetitive yield during the degradation, low reagent consumption, low maintenance requirements, and a degradative cycle time of only 50 min using a complete double cleavage program.
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The spectrophotometric determination of tyrosine and tryptophan in proteins.
TL;DR: (All right. re8erved)
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Multiple peptide composition of the large and small sub-units of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electrofocusing
TL;DR: In this article, a 14C-labeled fraction I protein was synthesized by Nicotiana tabacum leaves during 20 h of photosynthesis in the presence of 14CO2.
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Immobilized carboxypeptidase Y. Applications in protein chemistry.
TL;DR: After many unsuccessful attempts to prepare an immobilized CPY derivative exhibiting good operational stability over a wide pH range, it is found that hexamethylenediamine-Cl-Sepharose 4B (prepared by periodate axidation and reductive alkylation) is the support of choice.
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Atrax robustus envenomation.
TL;DR: Two patients who developed massive pulmonary oedema, profound vasoconstriction and hypertension followed by hypotension after Atrax Robustus envenomation are described.