Journal ArticleDOI
Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage
Alejandra Bernardini,Alejandra Bernardini,F. Hozbor,E. Sanchez,Miguel W. Fornés,Ricardo Alberio,Andreina Cesari +6 more
TLDR
It is concluded that SP proteins that bound to the sperm membrane were conserved among ram breeds, and that when added to frozen/thawed semen (along with an energy source), they repaired ram sperm damage and enhanced sperm motility.About:
This article is published in Theriogenology.The article was published on 2011-08-01. It has received 83 citations till now. The article focuses on the topics: Sperm plasma membrane & Sperm motility.read more
Citations
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Journal ArticleDOI
Seminal plasma proteins as modulators of the sperm function and their application in sperm biotechnologies.
I. Caballero,Inmaculada Parrilla,C. Almiñana,D. del Olmo,Jordi Roca,Emilio A. Martinez,Juan M. Vazquez +6 more
TL;DR: The current knowledge in the role of the major SP proteins as modulators of sperm functionality is reviewed and the possible applications of the SP proteins in sperm cryopreservation and flow cytometric sex-sorting are discussed.
Journal ArticleDOI
Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality
Patricia Peris-Frau,Ana J. Soler,María Iniesta-Cuerda,Alicia Martín-Maestro,Irene Sánchez-Ajofrín,Daniela Alejandra Medina-Chávez,María Rocío Fernández-Santos,Olga García-Álvarez,Alejandro Maroto-Morales,Vidal Montoro,José Julián Garde +10 more
TL;DR: The molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.
Journal ArticleDOI
Evolution and function of mammalian binder of sperm proteins
TL;DR: The current knowledge of BSP proteins strongly emphasizes their fundamental importance in male fertility and in the optimization of semen preservation techniques, making them crucial multifunctional proteins.
Journal ArticleDOI
Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma
Taylor Pini,T. Leahy,Clément Soleilhavoup,Guillaume Tsikis,Valérie Labas,Lucie Combes-Soia,Grégoire Harichaux,J.P. Rickard,Xavier Druart,Simon P. de Graaf +9 more
TL;DR: This work uses liquid chromatography-tandem mass spectrometry to investigate the proteome of ejaculated ram spermatozoa and presents the first evidence that despite its relatively complex proteomic composition, seminal plasma exposure leads to few novel proteins binding tightly to the ram sperm plasma membrane.
Journal ArticleDOI
Sublethal sperm freezing damage: Manifestations and solutions.
TL;DR: Several facets of sublethal freezing damage are explored, touching on cryocapacitation, the generation of reactive oxygen species and alterations to sperm proteins, lipids and sugars, and the cryoprotective potential of whole seminal plasma and individual proteins.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
The Physiology of Reproduction
Ernst Knobil,J. D. Neill +1 more
TL;DR: The gametes, fertilization and early embryogenesis the reproductive systems - the female, the male the pituitary and the hypothalmus, and the reproductive processes and their control.
Journal ArticleDOI
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
Journal ArticleDOI
Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon.
TL;DR: Successful semen cryopreservation enhances the advantages of AI over natural breeding, as long-term storage facilitates semen transport over distances, permits the quarantine of semen, and enables extended use of superior germplasm, even after the sire's death.
Journal ArticleDOI
A factor preventing the major head protein of bacteriophage T4 from random aggregation.
Ulrich K. Laemmli,Ulrich K. Laemmli,F. Beguin,F. Beguin,G. Gujer-Kellenberger,G. Gujer-Kellenberger +5 more
TL;DR: Temperature-shift experiments with ts -mutants in gene 31 demonstrate that the P23 aggregates can be dissolved by activated P31 and the dissolved P23 is normal, in that it can be used for incorporation into active phage.