Journal ArticleDOI
Construction and identification of cDNA clones for mouse ribosomal proteins: application for the study of r-protein gene expression.
Oded Meyuhas,Robert P. Perry +1 more
TLDR
Preliminary Southern blot analysis of Eco RI restriction fragments of mouse embryo DNA revealed very complex patterns, suggesting that a family of homologous sequences for the individual rp-mRNAs may exist in the mouse genome.About:
This article is published in Gene.The article was published on 1980-07-01. It has received 164 citations till now. The article focuses on the topics: Ribosomal protein & Recombinant DNA.read more
Citations
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Journal ArticleDOI
Arrest of the cell cycle by the tumour-suppressor BRCA1 requires the cdk-inhibitor p21waf1/cip1
Kumaravel Somasundaram,Huixi Violet Zhang,Yi Xin Zeng,Yariv Houvras,Yi Peng,Gen Sheng Wu,Jonathan D. Licht,Barbara L. Weber,Wafik S. El-Deiry +8 more
TL;DR: It is shown that BRCA1 transactivates expression of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 in a p53-independent manner, and that B RCA1 inhibits cell-cycle progression into the S-phase following its transfection into human cancer cells.
BookDOI
The nucleolus and ribosome biogenesis
TL;DR: In this paper, the authors describe the structure of preribosomes and their role in the pre-rRNA precoding process, and discuss the role of RNA polymerases in the precoding.
Journal ArticleDOI
Ischemic Preconditioning and Brain Tolerance: Temporal Histological and Functional Outcomes, Protein Synthesis Requirement, and Interleukin-1 Receptor Antagonist and Early Gene Expression
Frank C. Barone,Raymond F. White,Patricia A. Spera,Julie A. Ellison,R. William Currie,Xinkang Wang,Giora Z. Feuerstein +6 more
TL;DR: PC is a powerful inducer of ischemic brain tolerance as reflected by preservation of brain tissue and motor function and the importance of protein synthesis in PC-induced IT is evaluated.
Journal ArticleDOI
Two promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver
TL;DR: It is shown that two promoters of different strengths are involved in the tissue-specific expression of the alpha-amylase gene Amy-1a in the parotid gland and the liver of mouse.
Journal ArticleDOI
Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein
Fayez F. Safadi,Paul Thornton,Holly Magiera,Bruce W. Hollis,Michael A. Gentile,John G. Haddad,Stephen A. Liebhaber,Nancy E. Cooke +7 more
TL;DR: The role of DBP is to maintain stable serum stores of vitamin D metabolites and modulate the rates of its bioavailability, activation, and end-organ responsiveness, which may have evolved to stabilize and maintain serum levels of Vitamin D in environments with variable vitamin D availability.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Journal ArticleDOI
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
TL;DR: This method can be used to isolate clones of ColE1 hybrid plasmids that contain Drosophila melanogaster genes for 18 and 28S rRNAs and any gene whose base sequence is represented in an available RNA.
Journal ArticleDOI
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes
TL;DR: This procedure allows detection of specific RNA bands with high sensitivity and low background by hybridization with 32P-labeled DNA probes followed by autoradiography.
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Related Papers (5)
The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene
Kalin P. Dudov,Robert P. Perry +1 more
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Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family.
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