Journal ArticleDOI
Construction of a genetic linkage map for roses using RAPD and AFLP markers
Thomas Debener,L. Mattiesch +1 more
TLDR
The first linkage maps of the rose genome provide a tool for further genetic analyses of horticulturally important genes as, for example, resistance genes and a starting point for marker-assisted breeding in roses.Abstract:
A segregating population of diploid rose hybrids (2n = 2x = 14) was used to construct the first linkage maps of the rose genome. A total of 305 RAPD and AFLP markers were analysed in a population of 60 F1 plants based on a so-called ”double-pseudotestcross” design. Of these markers 278 could be located on the 14 linkage groups of the two maps, covering total map lengths of 326 and 370 cM, respectively. The average distances between markers in the maps for 93/1–117 and 93/1–119 is 2.4 and 2.6 cM, respectively. In addition to the molecular markers, genes controlling two phenotypic characters, petal number (double versus single flowers) and flower colour (pink versus white), were mapped on linkage groups 3 and 2, respectively. The markers closest to the gene for double flowers, Blfo, and to the gene for pink flower colour, Blfa, cosegregated without recombinants. The maps provide a tool for further genetic analyses of horticulturally important genes as, for example, resistance genes and a starting point for marker-assisted breeding in roses.read more
Citations
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Journal ArticleDOI
Multiple Models for Rosaceae Genomics
Vladimir Shulaev,Schuyler S. Korban,Bryon Sosinski,Albert G. Abbott,Herb S. Aldwinckle,Kevin M. Folta,Amy Iezzoni,Dorrie Main,Pere Arús,Abhaya M. Dandekar,Kim S. Lewers,Susan K. Brown,Thomas M. Davis,Susan E. Gardiner,Daniel Potter,Richard E. Veilleux +15 more
TL;DR: This report serves as a synopsis of the resources and initiatives of the Rosaceae community, recent developments in Rosaceae genomics, and plans to apply newly accumulated knowledge and resources toward breeding and crop improvement.
Journal ArticleDOI
Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits
L. Hibrand-Saint Oyant,Laurent Crespel,S. Rajapakse,S. Rajapakse,L. Zhang,L. Zhang,Fabrice Foucher +6 more
TL;DR: New microsatellites markers [simple sequence repeat (SSR)] have been isolated from rose and integrated into an existing amplified fragment-length polymorphism genetic map that was used to identify quantitative trait locus controlling date of flowering and number of petals.
Journal ArticleDOI
Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers.
TL;DR: It appears that thorn quantity is controlled by a major and a minor QTL which are located on the same linkage group at 36.5 and 3.2 cM from the single seasonal-blooming gene, respectively.
Journal ArticleDOI
Two genetic linkage maps of tetraploid roses
TL;DR: These first-generation maps provide initial tools for marker- assisted selection and gene introgression for the improvement of modern tetraploid roses.
Journal ArticleDOI
Construction of an integrated map of rose with AFLP, SSR, PK, RGA, RFLP, SCAR and morphological markers
Z. Yan,C. Denneboom,A. Hattendorf,Oene Dolstra,Thomas Debener,Piet Stam,P. B. Visser,P. B. Visser +7 more
TL;DR: The present linkage map is currently the most advanced map in rose with regard to marker density, genome coverage and with robust markers, giving good perspectives for QTL mapping and marker-assisted breeding in rose.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
A procedure for mapping Arabidopsis mutations using co‐dominant ecotype‐specific PCR‐based markers
TL;DR: A set of mapping markers have been designed for Arabidopsis thaliana that correspond to DNA fragments amplified by the polymerase chain reaction (PCR) that can be unambiguously mapped to one of the 10Arabidopsis chromosome arms in a single cross using a limited number of F2 progeny.
Journal ArticleDOI
High density molecular linkage maps of the tomato and potato genomes.
Steven D. Tanksley,Martin W. Ganal,James P. Prince,M. C. de Vicente,M. W. Bonierbale,Pierre Broun,T. M. Fulton,James J. Giovannoni,Silvana Grandillo,Gregory B. Martin +9 more
TL;DR: Currently tomato and potato are among the most thoroughly mapped eukaryotic species and the availability of high density molecular linkage maps should facilitate chromosome walking, quantitative trait mapping, marker-assisted breeding and evolutionary studies in these two important and well studied crop species.
Journal ArticleDOI
Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce
Ilan Paran,Richard W Michelmore +1 more
TL;DR: Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce, providing information on the molecular basis of RAPD markers.
Journal ArticleDOI
Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers
TL;DR: The combined use of RAPD markers and the pseudo-testcross configuration is proposed as a general strategy for the construction of single individual genetic linkage maps in outbred forest trees as well as in any highly heterozygous sexually reproducing living organisms.