Journal ArticleDOI
Continuous spore disruption using radially focused, high-frequency ultrasound.
Darrell P. Chandler,Jeremy Brown,Cynthia J. Bruckner-Lea,Lydia Olson,G. Jerry Posakony,Jennie R. Stults,and Nancy B. Valentine,Leonard J. Bond +7 more
TLDR
A novel, continuous-flow, radially focused ultrasonic disruptor capable of lysing Bacillus spores in the absence of added chemical denaturants, enzymes, or microparticles is reported on.Abstract:
We report on the development of a novel, continuous-flow, radially focused ultrasonic disruptor capable of lysing Bacillus spores in the absence of added chemical denaturants, enzymes, or microparticles. Greater than 99% disruption was achieved for Bacillus globigii spores and Escherichia coli and Bacillus subtilis vegetative cells with sample residence times of 62, 12, and 12 s, respectively. Microscopic and SEM images indicated that at equivalent power levels, the incidence of cell death or loss of viability typically exceeded the efficiency of (visible) cell lysis. However, semiquantitative PCR showed up to a 1000-fold increase in intracellular DNA availability from ultrasonically disrupted spores, and liberated DNA was intact and available for subsequent detection.read more
Citations
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Journal ArticleDOI
Cell disruption for microalgae biorefineries
Emre Günerken,Els D'Hondt,Michel H.M. Eppink,Linsey Garcia-Gonzalez,Kathy Elst,René H. Wijffels,René H. Wijffels +6 more
TL;DR: This review article focuses on the evaluation of conventional and emerging cell disruption technologies, and a comparison thereof with respect to their potential for the future microalgae biorefineries.
Journal ArticleDOI
Disruption of microalgal cells for the extraction of lipids for biofuels: Processes and specific energy requirements
TL;DR: An overview of microalgal cell disruption processes which are potentially suitable for large scale lipid extractions and the results show that further research and innovation is required for the sustainable cell disruption and lipid extraction from microalgae.
Journal ArticleDOI
Ultrasound increases the rate of bacterial cell growth.
William G. Pitt,S. Aaron Ross +1 more
TL;DR: It was found that low‐frequency ultrasound (70 kHz) of low acoustic intensity increased the growth rate of the cells compared to growth without ultrasound, however, at high intensity levels, cells were partially removed from the surface.
Journal ArticleDOI
Thermophilic bacilli and their importance in dairy processing.
TL;DR: A greater understanding of the structure and composition of thermophilic bacilli biofilms within the context of the milk processing environment and their link with spore formation is needed to develop better control measures.
Journal ArticleDOI
Perspective on optical biosensors and integrated sensor systems.
TL;DR: Examples of innovations are identified that will lead to smaller, faster, cheaper optical biosensor systems with capacity to provide effective and actionable information.
References
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PatentDOI
Expression monitoring by hybridization to high density oligonucleotide arrays
TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Journal ArticleDOI
Light-generated oligonucleotide arrays for rapid DNA sequence analysis
Ann Maria Caviani Pease,Dennis W. Solas,Edward Sullivan,Maureen T. Cronin,Christopher P. Holmes,Stephen P. A. Fodor +5 more
TL;DR: It is reported here how modern photolithographic techniques can be used to facilitate sequence analysis by generating miniaturized arrays of densely packed oligonucleotide probes, which can then be applied to parallel DNA hybridization analysis, directly yielding sequence information.
Journal ArticleDOI
DNA analysis and diagnostics on oligonucleotide microchips
Gennady Yershov,Victor Barsky,Alexander Belgovskiy,Eugene Kirillov,Edward Kreindlin,Igor Ivanov,Sergei Parinov,Dmitri Guschin,Aleksei Drobishev,Svetlana Dubiley,Andrei D. Mirzabekov +10 more
TL;DR: In this paper, the authors presented a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application to diagnostics for genetic diseases.
Journal ArticleDOI
Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil
Cheryl R. Kuske,Kaysie L. Banton,Dante L. Adorada,Peter C. Stark,Karen K. Hill,Paul J. Jackson +5 more
TL;DR: A small-scale procedure to rapidly extract and purify DNA from soils was developed for in-the-field use and found that combining a hot-detergent treatment with bead mill homogenization gave the highest DNA yields from all three microbial cell types and provided DNA from the broadest range of microbial groups in a natural soil community.
Journal ArticleDOI
A Minisonicator To Rapidly Disrupt Bacterial Spores for DNA Analysis
Phillip Belgrader,Derek J. Hansford,Gregory T. A. Kovacs,Kodumudi S. Venkateswaran,Raymond P. Mariella,Fred P. Milanovich,S Nasarabadi,Okuzumi M,Pourahmadi F,Northrup Ma +9 more
TL;DR: A minisonicator and prototype spore lysis cartridge were built to disrupt Bacillus spores in 30 s for rapid, real-time PCR analysis and improved PCR analysis by decreasing the limit of detection, reducing the time of detection and increasing the signal amplitude.