Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
Reads0
Chats0
TLDR
Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.Abstract:
The chloroplast, as the seat of chlorophyll pigments in plants, occupies a unique position in the economy of the green cell. In recent years there has been a renewed interest in the reactions and properties of chloroplasts as a result of the work of Hill (11, 12) and Hill and Scarisbrick (13, 14) who demonstrated that the reaction characteristic of photosynthesis in green plants, the evolution of oxygen, occurs in appreciable quantities in isolated chloroplasts under the influence of light and in the presence of suitable oxidants (2, 7, 8, 26). In the course of an investigation of oxygen evolution by isolated chloroplasts it was deemed important to explore their enzymatic composition. Of special interest were considered enzymes capable of participating in oxidation-reduction reactions, and more particularly, those localized principally, if not entirely, in the chloroplasts. This paper presents evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis.read more
Citations
More filters
Journal ArticleDOI
Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.).
TL;DR: This work provides a detailed insight into the mechanisms of drought response and defense in bread wheat from the perspective of phosphoproteomics, and identifies some important drought-tolerant candidates for further transgenosis study and incorporation into the breeding of resistant cultivars.
Journal ArticleDOI
Relationship between photoinhibition of photosynthesis, D1 protein turnover and chloroplast structure: effects of protein synthesis inhibitors
TL;DR: In excess (photoinhibitory) light at 20°C, turnover (coordinated degradation and synthesis) of D1 diminished the degree of photoinhibition, thought to be due to the accumulation of inactive photosystem II reaction centres still containing D1.
Journal ArticleDOI
Anthocyanin, Carotenoid, and Chlorophyll Changes in the Peel of Cox’s Orange Pippin Apples during Ripening on and off the Tree
TL;DR: The concentrations of various peel pigments of Cox's Orange Pippin apples have been measured during ripening on the tree and during storage at 12 °C as mentioned in this paper, and the carotenoids found in the unripe fruit were those characteristic of photosynthetic tissue, jS-carotene, lutein, violaxanthin, and neoxanthin.
Journal ArticleDOI
Polyphenol oxidase in leaves: is there any significance to the chloroplastic localization?
TL;DR: This review highlights the current understanding of the in vivo functions of PPO and considers the potential opportunities it presents for exploitation to increase stress tolerance in food crops.
Journal ArticleDOI
Red edge measurements for remotely sensing plant chlorophyll content
TL;DR: The feasibility of using the wavelength of the maximum slope of the red edge of leaf reflectance spectra, Lambda(re), as an indication of plant chlorophyll status was examined in the laboratory for single leaves of several species as discussed by the authors.
References
More filters
Journal ArticleDOI
Absorption of light by chlorophyll solutions
TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.
Journal ArticleDOI
Microelements in culture-solution experiments with higher plants
TL;DR: This paper is a report on the observed responses from a group of elements not generally credited with a function in plant nutrition, which suggested that these and other elements, if present in minute quantity, may favorably influence the growth of plants.
Journal ArticleDOI
Cytochrome and Respiratory Enzymes
TL;DR: The results of this study will enable to determine the nature of the relationship between the oxidising enzymes and the intracellular haematin compounds, and this will help to elucidate at least one portion of the complicated respiratory mechanism, of the cell.
Journal ArticleDOI
Oxygen Evolved by Isolated Chloroplasts
TL;DR: The hæmoglobin method, originally used by Hoppe-Seyler to demonstrate oxygen from green plants, has been applied to study the oxygen evolution of isolated chloroplasts exposed to light.
Journal ArticleDOI
Oxygen Produced by Isolated Chloroplasts
TL;DR: Inman (1935) brought further evidence as to the enzymic nature of the process, and showed also that fresh green extracts of many phanerogams will evolve oxygen in light, using the bacterial mathod.