Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
Reads0
Chats0
TLDR
Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.Abstract:
The chloroplast, as the seat of chlorophyll pigments in plants, occupies a unique position in the economy of the green cell. In recent years there has been a renewed interest in the reactions and properties of chloroplasts as a result of the work of Hill (11, 12) and Hill and Scarisbrick (13, 14) who demonstrated that the reaction characteristic of photosynthesis in green plants, the evolution of oxygen, occurs in appreciable quantities in isolated chloroplasts under the influence of light and in the presence of suitable oxidants (2, 7, 8, 26). In the course of an investigation of oxygen evolution by isolated chloroplasts it was deemed important to explore their enzymatic composition. Of special interest were considered enzymes capable of participating in oxidation-reduction reactions, and more particularly, those localized principally, if not entirely, in the chloroplasts. This paper presents evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis.read more
Citations
More filters
Journal ArticleDOI
Mechanism of photoinhibition: photochemical reaction center inactivation in system II of chloroplasts
TL;DR: A correlation of the kinetics of variable fluorescence yield loss with the inhibition of QA photoreduction suggested that photoinhibited reaction centers are incapable of generating a stable charge separation but are highly efficient in the trapping and non-photochemical dissipation of absorbed light.
Journal ArticleDOI
Spinach Thylakoid Polyphenol Oxidase : ISOLATION, ACTIVATION, AND PROPERTIES OF THE NATIVE CHLOROPLAST ENZYME
John H. Golbeck,Kirk Cammarata +1 more
TL;DR: A large seasonal variation in polyphenol oxidase activity may result from a decrease in enzyme content rather than inhibition of the enzyme present.
Journal ArticleDOI
XopD SUMO protease affects host transcription, promotes pathogen growth, and delays symptom development in xanthomonas-infected tomato leaves.
TL;DR: It is demonstrated that XopD, a type III effector from Xanthomonas campestris pathovar vesicatoria (Xcv), suppresses symptom production during the late stages of infection in susceptible tomato (Solanum lycopersicum) leaves.
Journal ArticleDOI
PHYCOBILISOMES OF PORPHYRIDIUM CRUENTUM : I. Isolation
E. Gantt,C. A. Lipschultz +1 more
TL;DR: Of the detergents tested, Triton X-100 gave the best results Freezing of the cells caused destruction of phycobilisomes, and preservation were optimal.
Journal ArticleDOI
Variability in leaf optical properties of Mesoamerican trees and the potential for species classification
TL;DR: In this paper, the authors examined the spectral reflectance of Mesoamerican trees for three applications: (1) to compare the magnitude of within and between-species variability in leaf reflectance, (2) to determine the potential for species identification, and (3) to test the strength of relationships between leaf traits (chlorophyll content, mesophyll attributes, thickness) and leaf spectral reflectances.
References
More filters
Journal ArticleDOI
Absorption of light by chlorophyll solutions
TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.
Journal ArticleDOI
Microelements in culture-solution experiments with higher plants
TL;DR: This paper is a report on the observed responses from a group of elements not generally credited with a function in plant nutrition, which suggested that these and other elements, if present in minute quantity, may favorably influence the growth of plants.
Journal ArticleDOI
Cytochrome and Respiratory Enzymes
TL;DR: The results of this study will enable to determine the nature of the relationship between the oxidising enzymes and the intracellular haematin compounds, and this will help to elucidate at least one portion of the complicated respiratory mechanism, of the cell.
Journal ArticleDOI
Oxygen Evolved by Isolated Chloroplasts
TL;DR: The hæmoglobin method, originally used by Hoppe-Seyler to demonstrate oxygen from green plants, has been applied to study the oxygen evolution of isolated chloroplasts exposed to light.
Journal ArticleDOI
Oxygen Produced by Isolated Chloroplasts
TL;DR: Inman (1935) brought further evidence as to the enzymic nature of the process, and showed also that fresh green extracts of many phanerogams will evolve oxygen in light, using the bacterial mathod.