Journal ArticleDOI
CRISPR/Cas12a-based photoelectrochemical sensing of microRNA on reduced graphene oxide-anchored Bi2WO6 coupling with catalytic hairpin assembly
TLDR
In this article , an innovative and generic CRISPR/Cas12a-driven photoelectrochemical (PEC) biosensing platform was developed for screening of microRNA-21 (miR-21) by coupling with target-triggered catalytic hairpin assembly (CHA) and reduced graphene oxide-anchored Bi 2 WO 6 (rGO-BWO) as the photoactive material.Abstract:
An innovative and generic CRISPR/Cas12a-driven photoelectrochemical (PEC) biosensing platform was developed for screening of microRNA-21 (miR-21) by coupling with target-triggered catalytic hairpin assembly (CHA) and reduced graphene oxide-anchored Bi 2 WO 6 (rGO-BWO) as the photoactive material. CHA isothermal amplification involved two programmable hairpin DNA modules and miR-21 as an activator. In the presence of miR-21, the products of target-triggered CHA circuit were inserted into the Cas12a-crRNA duplex to initial trans-cleavage capacity of CRISPR/Cas12a nuclease, accompanying the digestion on alkaline phosphatase (ALP)-labeled single-stranded DNA (ssDNA)-encoded magnetic bead (MB) through the activated CRISPR system. The ALPs were detached from magnetic beads and promoted the generation of ascorbic acid (AA), which increased the photocurrent of rGO-BWO-modified electrode. The value of photocurrent was positively proportional to the level of AA, which was also linearly correlated with target concentrations. Under optimum conditions, the CRISPR-based PEC sensing system displayed satisfying photocurrent responses toward miR-21 within the range from 1.0 fM to 1.0 nM with a limit of detection of 0.47 fM. In addition, the biosensor exhibited acceptable stability and excellent selectivity. Impressively, CHA-mediated CRISPR-based PEC biosensing platform provides a universal and sensitive method for clinical cancer diagnostics and biomolecular research. • An enhanced photoelectrochemical biosensor was designed for detection of microRNA. • Reduced graphene oxide-anchored Bi2WO6 used as the photoactive materials. • Bi2WO6-based carbon materials to enhance photoelectric conversion efficiency. • Catalytic hairpin assembly utilized for target recycling. • CRSIPR/Cas12 reaction system employed for in-situ amplified photocurrent. read more
Citations
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Journal ArticleDOI
Integrated solar-powered MEMS-based photoelectrochemical immunoassay for point-of-care testing of cTnI protein.
TL;DR: In this article , an artificial neural network-assisted solar-powered photoelectrochemical (SP-PEC) sensing platform for rapid screening of cardiac troponin I (cTnI) protein in the prognosis of patients with acute myocardial infarction (AMI) by integrating a selfpowered photoelectric signal output system with low-cost screen-printed paper electrodes functionalized with ultrathin Bi2O2S (BOS) nanosheets.
Journal ArticleDOI
Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21.
TL;DR: Wang et al. as discussed by the authors proposed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-mediated photoelectrochemical biosensors for the direct assay of miRNA-21.
Journal ArticleDOI
Recent advances and application of Bi2S3-based composites in photoelectrochemical sensors and biosensors
TL;DR: In this article , the authors have discussed the Bi2S3 semiconductor applied in PEC platform for sensing various target molecules and explored the challenges against Bi 2S3-based PEC and its future prospects.
Journal ArticleDOI
Bio-inspired polynorepinephrine based nanocoatings for reduced graphene oxide/gold nanoparticles composite for high-performance biosensing of Mycobacterium tuberculosis.
Neha Bisht,Monika Patel,Neeraj Dwivedi,Pradipta Kumar,D.P. Mondal,A.K. Srivastava,Chetna Dhand +6 more
TL;DR: In this article , polynorepinephrine (PNE) was used as a coating material for reduced graphene oxide (RGO) and gold nanoparticles (Au) in order to build an electrochemical genosensor for Mycobacterium tuberculosis (MTB) detection.
Journal ArticleDOI
A ratiometric fluorescent biosensor for rapid detection of Burkholderia pseudomallei by dual CRISPR/Cas12a trans-cleavage assisted signal enhancement
Shoujia Lin,Ying-Tzer Lin,Jie Wu,Guangming Li,Xiaotian Wu,Nini Luo,Wenting Li,Chuanlong Zhu,Rui Liu,Qiqi Xu,Qianfeng Xia,Huangxian Ju +11 more
TL;DR: In this paper , a ratiometric fluorescent sensor with dual recognition sites of target was designed for sensitive detection of Bp DNA, which was constructed with a TAMRA-labeled DNA as probe, which could adsorb on polymer dots (Pdots) at acidic pH to produce TAMRA fluorescence at the excitation wavelength of Pdots via fluorescence resonance energy transfer.
References
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Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
MicroRNAs in cancer: biomarkers, functions and therapy.
TL;DR: The potential applications of microRNAs for the clinical assessment of patient outcome in cancer, as well as in cancer monitoring and therapy are reviewed.
Journal ArticleDOI
Regulation of microRNA function in animals
Gebert Lfr,Ian J. MacRae +1 more
TL;DR: The mechanisms that modulate miRNA activity, stability and cellular localization through alternative processing and maturation, sequence editing, post-translational modifications of Argonaute proteins, subcellular localization and regulation of miRNA–target interactions are reviewed.
Journal ArticleDOI
The Role of Non-coding RNAs in Oncology
TL;DR: For decades, research into cancer biology focused on the involvement of protein-coding genes, but an explosion of studies into ncRNA biology has shown that they represent a diverse and prevalent group of RNAs, including both oncogenic molecules and those that work in a tumor suppressive manner.
Journal ArticleDOI
Recent Advances in Photoelectrochemical Sensing: From Engineered Photoactive Materials to Sensing Devices and Detection Modes.
Jian Shu,Dianping Tang +1 more
TL;DR: This review provides an over-view of the important advances in the construction and application of photoelectrochemical sensing in recent year and describes the typical strategies of design and engineering photoactive materials for improve the light excitation as well as charge separation/transfer to modulate the perfor-mance of photo Electrochemical sensing system.