Journal ArticleDOI
Crystals of fragment Fab': preparation from pepsin digests of human IgG myeloma proteins.
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TLDR
Crystals of fragment Fab, which has a molecular weight of 50,000 and contains the combining site of an antibody, may be of particular value because of its large molecular weight.Abstract:
INFORMATION concerning the three-dimensional structure of an antibody and the contours of its combining site can in theory be derived by X-ray crystallography. In view of the large molecular weight of antibody molecules (150,000 or greater), crystals of fragment Fab, which has a molecular weight of 50,000 and contains the combining site of an antibody, may be of particular value.read more
Citations
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Journal ArticleDOI
An analysis of the sequences of the variable regions of bence jones proteins and myeloma light chains and their implications for antibody complementarity
TL;DR: It is postulated that the information for site complementarity is contained in some extrachromosomal DNA such as an episome and is incorporated by insertion into the DNA of the structural genes for the variable region of short linear sequences of nucleotides.
Journal ArticleDOI
Three-Dimensional Structure of the Fab′ Fragment of a Human Immunoglobulin at 2.8-Å Resolution
TL;DR: The structure of the Fab' fragment of a human myeloma immunoglobulin was determined by x-ray crystallographic analysis at 2.8-A resolution and the Fourier map of the electron density was correlated with the aminoacid sequence to obtain a three-dimensional model.
Journal ArticleDOI
Structural invariants of antigen binding: comparison of immunoglobulin VL-VH and VL-VL domain dimers.
J Novotný,E Haber +1 more
TL;DR: The unusual mode of domain-domain association in the VL-VL dimer RHE correlates with its overall repulsive electrostatic energy, as opposed to negative energy values found in the domains of the other structures.
Journal ArticleDOI
A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A.
TL;DR: A new test principle for the detection of specific IgM‐class antibodies was developed and applied in an Enzyme‐Linked Immuno Sorbent Assay (ELISA) for the Detection of hepatitis A IgM antibodies.
Journal ArticleDOI
Structure of Fab' New at 6 A resolution.
TL;DR: The structure of human immunoglobulin Fab′ fragment has been elucidated at a resolution of 6 A and there are two globular subunits, which presumably correspond to the variable and constant regions of the heavy and light polypeptide chains of Immunoglobulins.
References
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Journal ArticleDOI
Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds.
TL;DR: The results indicate that the proteolytic enzyme splits off an inactive portion of the molecule; and that subsequent to this reaction the bivalent residue can be divided into two univalent fragments by breaking the disulfide bonds which hold them together.
Journal ArticleDOI
Crystallographic studies of a human immunoglobulin.
TL;DR: The first X-ray diffraction pictures of an intact immunoglobulin protein have been obtained and are reported to be the first of its kind.
Journal ArticleDOI
The interchain disulfide bonds of a human pathological immunoglobulin.
Steiner La,Porter Rr +1 more
Journal ArticleDOI
The covalent structure of a human gamma G-immunoglobulin. IV. The interchain disulfide bonds.
Journal ArticleDOI
Crystallization of Fragment Fab of human IgG myeloma proteins
Guido Rossi,Alfred Nisonoff +1 more