The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain
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This article is published in Biochemical Journal.The article was published on 1959-09-01 and is currently open access. It has received 2201 citations till now. The article focuses on the topics: Gamma globulin & Globulin.read more
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Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.
James S. Huston,D. Levinson,Meredith Mudgett-Hunter,M S Tai,Jirí Novotný,Michael N. Margolies,R J Ridge,Robert E. Bruccoleri,Edgar Haber,R Crea +9 more
TL;DR: A biosynthetic antibody binding site, which incorporated the variable domains of anti-digoxin monoclonal antibody 26-10 in a single polypeptide chain, was produced in Escherichia coli by protein engineering.
Journal ArticleDOI
Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors.
TL;DR: The ability of the hybridoma IgG to inhibit mouse FcRII was independent of the major histocompatibility complex and the monoclonal 2.4G2 IgG antigenic determinant was not present on rat, guinea pig, rabbit, or human F cR-bearing cells.
Journal ArticleDOI
Initiation of Platelet Adhesion by Arrest onto Fibrinogen or Translocation on von Willebrand Factor
TL;DR: Two distinct mechanisms initiating the adhesion of flowing platelets to thrombogenic surfaces are identified: glycoprotein Ib alpha binding to immobilized von Willebrand factor (vWF) and activated alpha IIb beta 3.
Journal ArticleDOI
Redistribution and Pinocytosis of Lymphocyte Surface Immunoglobulin Molecules Induced by Anti-Immunoglobulin Antibody
TL;DR: A possible mechanism for lymphocyte triggering by antigen is suggested and questions about cell membrane structure are raised.
Book ChapterDOI
Stability of proteins. Proteins which do not present a single cooperative system
TL;DR: The practical importance of thermodynamic studies of protein stability—that is, its importance not only for understanding the principles of organization of these molecules, but just for obtaining structural information on the domain level is emphasized.
References
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A colorimetric method for the determination of glucosamine and chondrosamine.
TL;DR: The acetylation of glucosamine which yields the Nmonoacetyl derivative, under the conditions described by Zuckerkandl and Messiner-Klebermass does not appear to be quantitative and rapid fading of the colour that develops on the addition of the p-dimethylaminobenzaldehyde reagent increases the difficulty of colorimetric estimation.
Journal ArticleDOI
Chromatography of amino acids on sulfonated polystyrene resins.
Stanford Moore,William H. Stein +1 more
TL;DR: In this article, the separation of amino acids by elution analysis on columns of synthetic ion exchange resins has been investigated and shown to have a resolving power comparable with that obtained with columns of starch or paper.
Journal ArticleDOI
Method for the determination of hexosamines in tissues.
TL;DR: During the course of investigations on the metabolism of hexosamines in animal tissues, absorption spectra of the color produced in the hexosamine method of Elson and Morgan revealed the presence of interfering chromogens.
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