Journal ArticleDOI
Cyclic ADP-ribose in insulin secretion from pancreatic beta cells
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TLDR
Results suggest that cADP-ribose is a mediator of calcium release from islet microsomes and may be generated in islets by glucose stimulation, serving as a second messenger for calcium mobilization in the endoplasmic reticulum.Abstract:
Inositol 1,4,5-trisphosphate (IP3) is thought to be a second messenger for intracellular calcium mobilization. However, in a cell-free system of islet microsomes, cyclic adenosine diphosphate-ribose (cADP-ribose), a nicotinamide adenine dinucleotide (NAD+) metabolite, but not IP3, induced calcium release. In digitonin-permeabilized islets, cADP-ribose and calcium, but not IP3, induced insulin secretion. Islet microsomes released calcium when combined with the extract from intact islets that had been incubated with high concentrations of glucose. Sequential additions of cADP-ribose inhibited the calcium release response to extracts from islets treated with high concentrations of glucose. Conversely, repeated additions of the islet extract inhibited the calcium release response to a subsequent addition of cADP-ribose. These results suggest that cADP-ribose is a mediator of calcium release from islet microsomes and may be generated in islets by glucose stimulation, serving as a second messenger for calcium mobilization in the endoplasmic reticulum.read more
Citations
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Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38
Maureen Howard,J C Grimaldi,J. F. Bazan,Frances E. Lund,Leopoldo Santos-Argumedo,R. M. E. Parkhouse,Timothy F. Walseth,Hon Cheung Lee +7 more
TL;DR: Soluble CD38 catalyzed the formation and hydrolysis of cadPR when added to NAD+.
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Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology
Fabio Malavasi,Silvia Deaglio,Ada Funaro,Enza Ferrero,Alberto L. Horenstein,Erika Ortolan,Tiziana Vaisitti,Semra Aydin +7 more
TL;DR: CD38 is a powerful disease marker for human leukemias and myelomas, is directly involved in the pathogenesis and outcome of human immunodeficiency virus infection and chronic lymphocytic leukemia, and controls insulin release and the development of diabetes.
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Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.
Minoru Takata,H. Sabe,Akiko Hata,T. Inazu,Yukio Homma,T. Nukada,Hirohei Yamamura,Tomohiro Kurosaki +7 more
TL;DR: It is demonstrated that Syk mediates IP3 generation, whereas Lyn regulates Ca2+ mobilization through a process independent of IP3generation, despite the normal kinetics ofIP3 turnover.
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The ryanodine receptor/calcium channel genes are widely and differentially expressed in murine brain and peripheral tissues.
TL;DR: It is reported that the three ryanodine receptor genes are expressed in almost all tissues analyzed, which suggests that RyRs may participate in the regulation of intracellular calcium homeostasis in a range of cells wider than previously recognized.
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PKA-Dependent and PKA-Independent Pathways for cAMP-Regulated Exocytosis
Susumu Seino,Tadao Shibasaki +1 more
TL;DR: Newly discovered cAMP-GEF/Epac mediates the PKA-independent effects on camp-regulated exocytosis, and localization of cAMP within intracellular compartments (cAMP compartmentation or compartmentalization) may be a key mechanism underlying the distinct effects of camp in different domains of the cell.
References
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