Cytokinin Regulates Type-A Arabidopsis Response Regulator Activity and Protein Stability via Two-Component Phosphorelay
Jennifer P.C. To,Jean Deruère,Bridey B. Maxwell,Veronica R. F. Morris,Claire E. Hutchison,Fernando Ferreira,G. Eric Schaller,Joseph J. Kieber +7 more
Reads0
Chats0
TLDR
The results indicate that phosphorylation of the receiver domain is required for type-A ARR function and suggest that negative regulation of cytokinin signaling by the type-B ARRs most likely involves phosphorylated-dependent interactions, and show that a subset of the type of ARR proteins are stabilized in response to cytokinIn in part via phosphorylations.Abstract:
The plant hormone cytokinin regulates many aspects of growth and development. Cytokinin signaling involves His kinase receptors that perceive cytokinin and transmit the signal via a multistep phosphorelay similar to bacterial two-component signaling systems. The final targets of this phosphorelay are a set of Arabidopsis thaliana Response Regulator (ARR) proteins containing a receiver domain with a conserved Asp phosphorylation site. One class of these, the type-A ARRs, are negative regulators of cytokinin signaling that are rapidly transcriptionally upregulated in response to cytokinin. In this study, we tested the role of phosphorylation in type-A ARR function. Our results indicate that phosphorylation of the receiver domain is required for type-A ARR function and suggest that negative regulation of cytokinin signaling by the type-A ARRs most likely involves phosphorylation-dependent interactions. Furthermore, we show that a subset of the type-A ARR proteins are stabilized in response to cytokinin in part via phosphorylation. These studies shed light on the mechanism by which type-A ARRs act to negatively regulate cytokinin signaling and reveal a novel mechanism by which cytokinin controls type-A ARR function.read more
Citations
More filters
Journal ArticleDOI
Hormone Crosstalk in Plant Disease and Defense: More Than Just JASMONATE-SALICYLATE Antagonism
TL;DR: Recent advances are reported, updating current knowledge on classical defense hormones SA, JA, and ET, and the roles of auxin, abscisic acid (ABA), cytokinins (CKs), and brassinosteroids in molding plant-pathogen interactions are updated.
Journal ArticleDOI
Cytokinin action in plant development
Tomáš Werner,Thomas Schmülling +1 more
TL;DR: This work reviews recent progress in understanding the cytokinin system and its links to the regulatory pathways that respond to internal and external signals and contributes to the fine-tuning of quantitative growth regulation in plants.
Journal ArticleDOI
Cytokinin Signaling Networks
TL;DR: Highlights focus on the integration of cytokinin signaling components into regulatory networks in specific contexts, ranging from molecular, cellular, and developmental regulations in the embryo, root apical meristem, stem and root vasculature, and nodule organogenesis to organismal responses underlying immunity, stress tolerance, and senescence.
Journal ArticleDOI
Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis
TL;DR: This study demonstrates that ethylene negatively regulates cold signaling at least partially through the direct transcriptional control of cold-regulated CBFs and type-A ARR genes by EIN3, and provides evidence that type- A ARRs function as key nodes to integrate ethylene and cytokinin signaling in regulation of plant responses to environmental stress.
Journal ArticleDOI
The plant vascular system: evolution, development and functions
William J. Lucas,Andrew Groover,Raffael Lichtenberger,Kaori Miyashima Furuta,Shri Ram Yadav,Ykä Helariutta,Xin Qiang He,Hiroo Fukuda,Julie Kang,Siobhan M. Brady,John W. Patrick,John S. Sperry,Akiko Yoshida,Ana Flor López-Millán,Michael A. Grusak,Pradeep Kachroo +15 more
TL;DR: The evolutionary events that gave rise to the tracheophytes are examined, followed by analysis of the genetic and hormonal networks that cooperate to orchestrate vascular development in the gymnosperms and angiosperms, in a comprehensive picture of the state-of-the-art in the area of plant vascular biology.
References
More filters
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
TL;DR: Development and application of REST is explained, the usefulness of relative expression in real-time PCR using REST is discussed and the mathematical model used is based on the PCR efficiencies and the mean crossing point deviation between the sample and control group.
Journal ArticleDOI
Two-component signal transduction
TL;DR: Detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins, which consists of two conserved components, a histidine protein kinase and a response regulator protein.
Journal ArticleDOI
CYTOKININS: Activity, Biosynthesis, and Translocation
TL;DR: Recent findings on the relationship between CK structural variation and activity, distinct features in CK biosynthesis between higher plants and Agrobacterium infected plants, CK translocation at whole-plant and cellular levels, and CKs as signaling molecules for nutrient status via root-shoot communication are summarized.
Journal ArticleDOI
A glucocorticoid-mediated transcriptional induction system in transgenic plants
Takashi Aoyama,Nam-Hai Chua +1 more
TL;DR: The results indicate that a stringent chemical control of transcription can be achieved in plants with the GVG system, and can also be regulated by DEX in transgenic Arabidopsis plants.