Journal ArticleDOI
Deconvolution of fluorescence decay curves. A critical comparison of techniques
About:
This article is published in The Journal of Physical Chemistry.The article was published on 1979-05-01. It has received 295 citations till now. The article focuses on the topics: Deconvolution & Convolution.read more
Citations
More filters
Journal ArticleDOI
Exponential analysis in physical phenomena
Andrei A. Istratov,O. F. Vyvenko +1 more
TL;DR: In this paper, the authors discuss the fundamental mathematical limitations of exponential analysis, outlines the critical aspects of acquisition of exponential transients for subsequent analysis, and gives a comprehensive overview of numerical algorithms used in exponential analysis.
Journal ArticleDOI
Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy
Noël Boens,Wenwu Qin,Nikola Basarić,Johan Hofkens,Marcel Ameloot,Jacques Pouget,Jean Pierre Lefèvre,Bernard Valeur,Enrico Gratton,Martin vandeVen,Martin vandeVen,Norberto D. Silva,Yves Engelborghs,K Willaert,Alain Sillen,Garry Rumbles,David Phillips,Antonie J. W. G. Visser,Arie van Hoek,Joseph R. Lakowicz,Henryk Malak,Ignacy Gryczynski,Arthur G. Szabo,Don T. Krajcarski,Naoto Tamai,Atsushi Miura +25 more
TL;DR: A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source.
Journal ArticleDOI
The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
TL;DR: In this article, the authors quantified the extent of sample heterogeneity in a melange of fluorophores and found that the heterogeneity arises from solvent-fluorophore interactions or from the fluorophore per se.
Time-Correlated Single Photon Counting
TL;DR: Time-resolved fluorescence spectroscopy is a powerful analysis tool in fundamental physics as well as in the life sciences as mentioned in this paper, and it can be implemented in the time domain by recording the time dependent intensity profile of the emitted light upon excitation by a short flash of light.
Journal ArticleDOI
Analysis of time-resolved fluorescence anisotropy decays.
TL;DR: A new method was proposed in which parallel- and perpendicular-polarized fluorescence curves were fit simultaneously, which takes full advantage of the statistical properties of the measured curves and is shown to be more sensitive than other methods to systematic errors present in the data.