Journal ArticleDOI
Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes.
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TLDR
Six established lines from human squamous cell carcinomas are examined for defects in the triggering of terminal differentiation and the phenotype of increased survival in semi-solid medium may be used to detect and select malignantly transformed keratinocytes.About:
This article is published in Cell.The article was published on 1980-11-01. It has received 322 citations till now.read more
Citations
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Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
TL;DR: The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.
Journal ArticleDOI
Calcium-Regulated Differentiation of Normal Human Epidermal Keratinocytes in Chemically Defined Clonal Culture and Serum-Free Serial Culture
Steven T. Boyce,Richard G. Ham +1 more
TL;DR: High-voltage electron microscopy after detergent extraction of human epidermal keratinocyte (HK) colonies grown in the defined medium with low and high calcium has revealed specific changes in the intermediate filament network and keratohyalin granules corresponding to changes in cellular differentiation.
Journal ArticleDOI
Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts
TL;DR: DNAs obtained from human, rabbit and mouse bladder carcinomas lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection.
Journal Article
Tumorigenic Keratinocyte Lines Requiring Anchorage and Fibroblast Support Cultured from Human Squamous Cell Carcinomas
TL;DR: It is demonstrated that SCC's often grow as established lines in culture, but they frequently possess in vitro growth requirements similar to those of normal keratinocytes.
Journal ArticleDOI
Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation
TL;DR: Results demonstrate that expression of the myc and rasKi proto-oncogenes is dependent upon the cellular growth state, and that growth control exhibits growth-factor-dependent, cell-cycle-timed oncogene expression.
References
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Journal ArticleDOI
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
James G. Rheinwatd,Howard Green +1 more
TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
Journal ArticleDOI
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines
George J. Todaro,Howard Green +1 more
TL;DR: Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending on the inoculation density, but nearly all cultures developed into established lines within 3 months of culture.
Journal ArticleDOI
Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma
James G. Rheinwald,Howard Green +1 more
TL;DR: From a transplantable mouse teratoma it has been possible to derive an established keratinizing cell line (XB) which grows well in cultures containing lethally irradiated 3T3 fibroblasts at the correct density.
Journal ArticleDOI
Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes
James G. Rheinwald,Howard Green +1 more
TL;DR: The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermis growth factor, a polypeptide mitogen, which seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation.
Journal ArticleDOI
Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions.
Robert H. Rice,Howard Green +1 more
TL;DR: Late in the terminal differentiation of epidermis and cultured epidermal cells, a protein envelope located beneath the plasma membrane becomes cross-linked by cellular transglutaminase, and cannot be extracted from keratinocytes after their envelopes have become cross- linked.
Related Papers (5)
Tumorigenic Keratinocyte Lines Requiring Anchorage and Fibroblast Support Cultured from Human Squamous Cell Carcinomas
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
James G. Rheinwatd,Howard Green +1 more