Journal ArticleDOI
Determination of iduronic acid and glucuronic acid in glycosaminoglycans after stoichiometric reduction and depolymerization using high-performance liquid chromatography and ultraviolet detection
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TLDR
The reduction of uronic acids in glycosaminoglycans prior to depolymerization reactions is one way in which the uronic acid content of polysaccharides can be studied without major losses.About:
This article is published in Analytical Biochemistry.The article was published on 1988-08-01. It has received 73 citations till now. The article focuses on the topics: Depolymerization & Iduronic acid.read more
Citations
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Journal ArticleDOI
Derivatization of carbohydrates for chromatographic, electrophoretic and mass spectrometric structure analysis.
TL;DR: This review article gives an overview of derivatization methods of carbohydrates for their liquid chromatographic and electrophoretic separation, as well as the mass spectrometric characterization.
Journal ArticleDOI
Human platelet heparanase: purification, characterization and catalytic activity.
TL;DR: Human platelet heparanase has now been purified 1700-fold to homogeneity in 19% yield by a five column procedure, suggesting that sulphated polysaccharides which inhibit tumour metastasis may act to prevent both tumour cell and platelets degradation of endothelial cell surface HS and the basal laminar.
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Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii
TL;DR: In this article, the authors used three-factor-three-level Box-Behnken design and response surface methodology based on single-factor experiments to extract polysaccharides from Hyriopsis cumingii (HCPS).
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Pancreatic carcinoma is characterized by elevated content of hyaluronan and chondroitin sulfate with altered disaccharide composition
Achilleas D. Theocharis,Marina E. Tsara,Nikoletta Papageorgacopoulou,Dionisis D. Karavias,Dimitrios A. Theocharis +4 more
TL;DR: It is indicated, for the first time, that human pancreatic carcinoma is characterized by highly increased amounts of hyaluronan and of a structurally altered CS.
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Altered content composition and structure of glycosaminoglycans and proteoglycans in gastric carcinoma
Achilleas D. Theocharis,Demitrios H. Vynios,N. Papageorgakopoulou,Spyros S. Skandalis,Dimitrios A. Theocharis +4 more
TL;DR: HGC contained about 2-fold increased amounts of GAGs in comparison to HNG, and the accumulation of core proteins of versican and decorin in HGC was also associated with many post-translational modifications, referring to the number, size, degree and patterns of sulphation and epimerization of CS/DS chains.
References
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Journal ArticleDOI
Method for the determination of hexosamines in tissues.
TL;DR: During the course of investigations on the metabolism of hexosamines in animal tissues, absorption spectra of the color produced in the hexosamine method of Elson and Morgan revealed the presence of interfering chromogens.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.
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Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups.
R. L. Taylor,H. E. Conrad +1 more
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Determination of glycosaminoglycans (mucopolysaccharides) from tissues on the microgram scale
TL;DR: Application of the procedure to histological sections of the intervertebral disc and the nasal septum cartilage revealed differences in the amount and properties of glycosaminoglycans in tissue structures 100–200 μ apart, and the method should be useful to study the distribution of gly cosaminoglycan-cetylpyridinium complexes at the tissue level.