Journal ArticleDOI
Development of a novel multiplex PCR assay for the identification of Salmonella enterica Typhimurium and Enteritidis
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TLDR
The results demonstrate that the comparative genomic method is a valuable tool for identifying new specific targets, a necessary step for developing rapid and accurate detection methods for foodborne pathogens.About:
This article is published in Food Control.The article was published on 2012-09-01. It has received 55 citations till now. The article focuses on the topics: Multiplex polymerase chain reaction & Salmonella enterica.read more
Citations
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Molecular methods for serovar determination of Salmonella
TL;DR: In this paper, the authors reviewed reported methodologies for Salmonella molecular serotyping and determined the "serovar-prediction accuracy", as the percentage of isolates for which the serovar was correctly classified by a given method.
Journal ArticleDOI
Developing a novel immunochromatographic test strip with gold magnetic bifunctional nanobeads (GMBN) for efficient detection of Salmonella choleraesuis in milk
TL;DR: In this article, gold magnetic bifunctional nanobeads (GMBN) were selected as a novel label to prepare immunochromatographic test strip for detection of Salmonella choleraesuis.
Journal ArticleDOI
Simultaneous detection of Listeria species isolated from meat processed foods using multiplex PCR
Jio Ryu,Si Hong Park,Yeon Seok Yeom,Anupama Shrivastav,Soon-Ho Lee,Young-Rok Kim,Hae-Yeong Kim +6 more
TL;DR: This established multiplex PCR provides rapid and reliable results and will be useful for the detection of Listeria species in contaminated food products and clinical samples.
Journal ArticleDOI
Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR.
Raymond Heymans,Amir Vila,Caroliene A. M. van Heerwaarden,Claudia C. C. Jansen,Greetje A.A. Castelijn,Menno van der Voort,Elisabeth G. Biesta-Peters +6 more
TL;DR: The multiplex qPCR assay developed allows for rapid screening of Salmonella spp.
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A colorimetric assay method for invA gene of Salmonella using DNAzyme probe self-assembled gold nanoparticles as single tag
Rong Luo,Yahui Li,Xiaojuan Lin,Fang Dong,Wei Zhang,Li Yan,Wei Cheng,Huangxian Ju,Huangxian Ju,Shijia Ding +9 more
TL;DR: A simple colorimetric sensing method developed for detection of invA gene of Salmonella by combining DNAzyme probe self-assembled gold nanoparticles and polymerase chain reaction (PCR) technique exhibited good stability, sensitivity and simplicity.
References
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Journal ArticleDOI
Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard
TL;DR: A simple PCR assay that is specific for Salmonella spp.
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A 6×6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli
TL;DR: A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter.
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Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China
Baowei Yang,Dong Qu,Xiuli Zhang,Jinling Shen,Shenghui Cui,Ying Shi,Meili Xi,Min Sheng,Shuai Zhi,Jianghong Meng,Jianghong Meng +10 more
TL;DR: The findings indicated that Salmonella contamination was common in retail meats, and that the Salmoneella isolates were phenotypically and genetically diverse.
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Salmonella contamination: a significant challenge to the global marketing of animal food products
L. Plym Forshell,M. Wierup +1 more
TL;DR: The authors conclude that the key to controlling Salmonella is to follow the general rules that have been successfully applied to other infectious diseases, and focus on a range of preventive actions.
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Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples
Juan Carlos Alvarez,Mertxe Sota,Ana Belén Vivanco,Ildefonso Perales,Ramón Cisterna,Aitor Rementeria,Javier Garaizar +6 more
TL;DR: A multiplex PCR assay for Salmonella detection and epidemiological typing was developed and was reproducible for all serotypes studied, apart from some C2 serogroup strains.