Journal ArticleDOI
Development of the system ensuring a high-level expression of hepatitis C virus nonstructural NS5B and NS5A proteins.
Alexander V. Ivanov,A. N. Korovina,Vera L. Tunitskaya,Dmitry A. Kostyuk,Vladimir O. Rechinsky,Marina K. Kukhanova,Sergey N. Kochetkov +6 more
TLDR
The plasmid pET-21d-2c-5BDelta55 effectively expressing a C-terminally truncated form of the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp) was constructed and a 29-fold enhancement of the protein yield was accompanied by only a 2.5-fold increase of the corresponding mRNA level.About:
This article is published in Protein Expression and Purification.The article was published on 2006-07-01. It has received 51 citations till now. The article focuses on the topics: NS5B & Protein A/G.read more
Citations
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Journal ArticleDOI
Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements.
TL;DR: The current technology used for commercial production of recombinant therapeutics in E. coli is summarized and recent advances that can potentially expand the use of this system toward more sophisticated protein therapeutics are summarized.
Journal ArticleDOI
Identification and evaluation of anti hepatitis C virus phytochemicals from Eclipta alba.
TL;DR: Eclipta alba extract and phytochemicals isolated from active fractions display anti-HCV activity in vitro and in cell culture system and can be used as an effective alternative and complementary treatment against HCV.
Journal ArticleDOI
The design, synthesis and biological evaluations of C-6 or C-7 substituted 2-hydroxyisoquinoline-1,3-diones as inhibitors of hepatitis C virus.
Yue-Lei Chen,Jing Tang,Matthew J. Kesler,Yuk Y. Sham,Robert Vince,Robert J. Geraghty,Zhengqiang Wang +6 more
TL;DR: The design, chemical synthesis, replicon and biochemical assays, and molecular docking of C-6 or C-7 aryl substituted 2-hydroxyisoquinoline-1,3-diones as novel HCV inhibitors are described and it is suggested that these inhibitors may bind to the NS5B active site.
Journal ArticleDOI
A cell-based reporter assay for inhibitor screening of hepatitis C virus RNA-dependent RNA polymerase
TL;DR: The BHK-NS5B-FRLuc reporter cell line is created that carries stably transfected NS5B and a bicistronic reporter gene, (+)FLuc-(-)UTR-RLuc, which can be used to simultaneously measure cellular toxicity and intracellular RdRp activity.
Journal ArticleDOI
1-[2-(2-Benzoyl- and 2-benzylphenoxy)ethyl]uracils as potent anti-HIV-1 agents.
Mikhail S. Novikov,Olga N. Ivanova,Alexander V. Ivanov,Alexander Ozerov,Vladimir T. Valuev-Elliston,Kartik Temburnikar,G. V. Gurskaya,Sergey N. Kochetkov,Christophe Pannecouque,Jan Balzarini,Katherine L. Seley-Radtke +10 more
TL;DR: Evaluation revealed that the inhibitors were active against most nevirapine-resistant mono- and di-substituted RTs with the exception of the V106A RT, and can be regarded as potential lead compounds against the wild-type virus and drug-resistant forms.
References
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Journal ArticleDOI
Recombinant protein expression in Escherichia coli.
TL;DR: Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, together with serendipitous discoveries and the availability of improved genetic tools are making this bacterium more valuable than ever for the expression of complex eukaryotic proteins.
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Codon usage tabulated from international DNA sequence databases: status for the year 2000
TL;DR: The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database.
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Strategies for achieving high-level expression of genes in Escherichia coli.
TL;DR: Progress in the understanding of several biological processes promises to broaden the usefulness of Escherichia coli as a tool for gene expression and the remarkable increase in the availability of fusion partners offers a wide range of tools for improved protein folding, solubility, protection from proteases, yield, and secretion into the culture medium.
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Evidence for two functional gal promoters in intact Escherichia coli cells.
TL;DR: The results strongly suggest that the same two overlapping promoters, which were previously identified by in vitro transcription of gal DNA fragments, also control the expression of the galactose operon in intact cells.
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Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus.
TL;DR: Experimental evidence that an RNA‐dependent RNA polymerase is encoded by HCV and that this enzymatic activity is the function of the 65 kDa non‐structural protein 5B (NS5B) is presented, representing a first important step towards a better understanding of the life cycle of the HCV.