Direct reprogramming of mouse fibroblasts to cardiomyocyte-like cells using Yamanaka factors on engineered poly(ethylene glycol) (PEG) hydrogels.
Amanda W. Smith,Jake D. Hoyne,Peter K. Nguyen,Dylan A. McCreedy,Haytham Aly,Igor R. Efimov,Stacey Rentschler,Donald L. Elbert +7 more
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TLDR
PEG hydrogels improved proliferation and reprogramming efficiency and designed poly(ethylene glycol) culture substrates to promote MEF adhesion via laminin and RGD-binding integrins to illustrate that cellular reprograming may be enhanced using custom-engineered materials.About:
This article is published in Biomaterials.The article was published on 2013-09-01 and is currently open access. It has received 41 citations till now. The article focuses on the topics: Reprogramming.read more
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Hydrogel as a bioactive material to regulate stem cell fate
TL;DR: The varying types of the hydrogels and stem cells, which are most commonly used in the biomedical fields, are discussed and further investigate how Hydrogels interact with stem cells from the perspective of their biomedical application, while providing insights into the design and development of hydrogel for drug delivery, tissue engineering and regenerative medicine purpose.
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Direct reprogramming of adult cells: avoiding the pluripotent state.
TL;DR: The procedure of using mature, fully differentiated cells and inducing them toward other cell types while bypassing an intermediate pluripotent state is termed direct reprogramming, which could be then directly implemented in regenerative and personalized medicine.
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Polysaccharide matrices used in 3D in vitro cell culture systems.
TL;DR: This review describes how these naturally sourced biomaterials satisfy several key properties for current 3D cell culture needs and can also be synthetically modified or blended with additional components to tailor their cell engagement properties.
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Substrate elasticity regulates adipose-derived stromal cell differentiation towards osteogenesis and adipogenesis through β-catenin transduction.
TL;DR: The importance of stiffness on ASC differentiation is indicated and could help to increase understanding of the mechanism underlying molecular signal transduction from mechanosensing, mechanotransducing to stem cell differentiation.
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Survival, differentiation, and migration of high-purity mouse embryonic stem cell-derived progenitor motor neurons in fibrin scaffolds after sub-acute spinal cord injury
Dylan A. McCreedy,Thomas Wilems,Hao Xu,Jessica C. Butts,Chelsea R. Brown,Amanda W. Smith,Shelly E. Sakiyama-Elbert +6 more
TL;DR: The survival and differentiation of enriched ES cell-derived progenitor motor neurons (pMNs) within three dimensional (3D) fibrin scaffolds in vitro and when transplanted into a sub-acute dorsal hemisection model of SCI into neurons, oligodendrocytes and astroCytes are demonstrated.
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Analyzing real-time PCR data by the comparative C(T) method.
TL;DR: This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Heart Disease and Stroke Statistics—2010 Update A Report From the American Heart Association
Donald M. Lloyd-Jones,Robert J. Adams,Todd M. Brown,Mercedes R. Carnethon,Shifan Dai,Giovanni de Simone,T. Bruce Ferguson,Earl S. Ford,Karen L. Furie,Cathleen Gillespie,Alan S. Go,Kurt J. Greenlund,Nancy Haase,Susan M. Hailpern,P. Michael Ho,Virginia J. Howard,Brett M. Kissela,Steven J. Kittner,Daniel T. Lackland,Lynda D. Lisabeth,Ariane Marelli,Mary M. McDermott,James B. Meigs,Dariush Mozaffarian,Michael E. Mussolino,Graham Nichol,Véronique L. Roger,Wayne D. Rosamond,Ralph L. Sacco,Paul D. Sorlie,Randall S. Stafford,Thomas Thom,Sylvia Wasserthiel-Smoller,Nathan D. Wong,Judith Wylie-Rosett +34 more
TL;DR: The Statistical Update brings together the most up-to-date statistics on heart disease, stroke, other vascular diseases, and their risk factors and presents them in its Heart Disease and Stroke Statistical Update each year.
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Executive Summary: Heart Disease and Stroke Statistics—2010 Update
Donald M. Lloyd-Jones,Robert J. Adams,Todd M. Brown,Mercedes R. Carnethon,Shifan Dai,Giovanni de Simone,T. Bruce Ferguson,Earl S. Ford,Karen L. Furie,Cathleen Gillespie,Alan S. Go,Kurt J. Greenlund,Nancy Haase,Susan M. Hailpern,P. Michael Ho,Virginia J. Howard,Brett M. Kissela,Steven J. Kittner,Daniel T. Lackland,Lynda D. Lisabeth,Ariane Marelli,Mary M. McDermott,James B. Meigs,Dariush Mozaffarian,Michael E. Mussolino,Graham Nichol,Véronique L. Roger,Wayne D. Rosamond,Ralph L. Sacco,Paul D. Sorlie,Randall S. Stafford,Thomas Thom,Sylvia Wasserthiel-Smoller,Nathan D. Wong,Judith Wylie-Rosett +34 more
TL;DR: Each year, the American Heart Association brings together the most up-to-date statistics on heart disease, stroke, other vascular diseases, and their risk factors and presents them in its Heart Disease and Stroke Statistical Update.