Journal ArticleDOI
Discovery of a novel putative atypical porcine pestivirus in pigs in the USA.
Ben M. Hause,Emily A. Collin,Lalitha Peddireddi,Fangfeng Yuan,Zhenhai Chen,Richard A. Hesse,Phillip C. Gauger,Travis Clement,Ying Fang,Gary A. Anderson +9 more
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TLDR
The molecular and serological results suggest that APPV is a novel, highly divergent porcine pestivirus widely distributed in US pigs.Abstract:
Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding a predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that of a recently partially characterized Rhinolophus affinis pestivirus (RaPV) and approximately 25-28 % pairwise identity to those of other pestiviruses. The virus was provisionally named atypical porcine pestivirus (APPV). Metagenomic sequencing of 182 serum samples identified four additional APPV-positive samples. Positive samples originated from five states and ELISAs using recombinant APPV Erns found cross-reactive antibodies in 94 % of a collection of porcine serum samples, suggesting widespread distribution of APPV in the US swine herd. The molecular and serological results suggest that APPV is a novel, highly divergent porcine pestivirus widely distributed in US pigs.read more
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A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure
Rachel Palinski,Pablo Piñeyro,Pengcheng Shang,Fangfeng Yuan,Rui Guo,Ying Fang,Emily Byers,Ben M. Hause +7 more
TL;DR: Results suggest that PCV3 commonly circulates within U.S. swine and may play an etiologic role in reproductive failure and PDNS and further studies are needed to elucidate its significance and role in PCVAD.
Journal ArticleDOI
Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae.
Donald B. Smith,Donald B. Smith,Gregor Meyers,Jens Bukh,Ernest A. Gould,Thomas P. Monath,A. Scott Muerhoff,Alexander G. Pletnev,Rebecca Rico-Hesse,Jack T. Stapleton,Peter Simmonds,Paul Becher +11 more
TL;DR: Seven new species in the genus Pestivirus (family Flaviviridae) are proposed in addition to the four existing species, and naming species in a host-independent manner using the format Pestvirus X.
Journal ArticleDOI
Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus
TL;DR: The highest number of various BVDV subgenotypes has been documented in European countries, indicating greater genetic diversity of the virus on this continent.
Journal ArticleDOI
Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence.
Lukas Schwarz,Christiane Riedel,Sandra Högler,Leonie J. Sinn,Thomas Voglmayr,Bettina Wöchtl,Nora Dinhopl,Barbara Rebel-Bauder,Herbert Weissenböck,Andrea Ladinig,Till Rümenapf,Benjamin Lamp +11 more
TL;DR: Appetical porcine pestivirus genomes were detected continuously in piglets that gradually recovered from CT, while the antibody titers decreased over a 12-week interval, pointing towards maternally transmitted antibodies.
Journal ArticleDOI
Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor
Alexander Postel,Florian Hansmann,Christine Baechlein,Nicole Fischer,Malik Alawi,Adam Grundhoff,Sarah Derking,Jörg Tenhündfeld,Vanessa M. Pfankuche,Vanessa Herder,Wolfgang Baumgärtner,Michael Wendt,Paul Becher +12 more
TL;DR: The results of this study strongly suggest that the presence of APPV genomes in newborn piglets correlates with CT, while no association with clinical disease could be observed in viremic adult pigs.
References
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MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: An advanced version of the Molecular Evolutionary Genetics Analysis software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, is released, which enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny.
Brief Communication MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: The Molecular Evolutionary Genetics Analysis (MEGA) software as discussed by the authors provides facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, including the inference of timetrees.
Journal ArticleDOI
Processing of the envelope glycoproteins of pestiviruses.
TL;DR: Using pulse-chase experiments, it is shown that a pestiviral glycoprotein precursor, E012, is formed that is processed into E0, E1, and E2 in an ordered fashion, and comparison of the sequences around the cleavage sites of pestiviruses suggests a general processing scheme for the structural glycoproteins.
Journal ArticleDOI
Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification
Paul Becher,Ramiro Avalos Ramirez,Michaela Orlich,Sibilina Cedillo Rosales,Matthias König,Matthias Schweizer,Hanspeter Stalder,Horst Schirrmeier,Heinz-Jürgen Thiel +8 more
TL;DR: Taking into account the host origin, the lack of differences concerning the course of disease, and the results of the genetic and antigenic analyses, it is suggested that BDV-1, BDv-2, andBDV-3 should be considered as major genotypes within the species BDV.
Journal ArticleDOI
Detection of Zoonotic Pathogens and Characterization of Novel Viruses Carried by Commensal Rattus norvegicus in New York City
Cadhla Firth,Meera Bhat,Matthew A. Firth,Simon H. Williams,Matthew J. Frye,Peter Simmonds,Juliette M. Conte,James Ng,Joel A. Garcia,Nishit Bhuva,Bohyun Lee,Xiaoyu Che,Phenix-Lan Quan,W. Ian Lipkin +13 more
TL;DR: The findings indicate that urban rats are reservoirs for a vast diversity of microbes that may affect human health and indicate a need for increased surveillance and awareness of the disease risks associated with urban rodent infestation.