Distribution of iron-containing superoxide dismutase in vascular plants.
Susan M. Bridges,Marvin L. Salin +1 more
TLDR
Of the 43 plant families investigated, the Fe-containing superoxide dismutase was found in three families: Gingkoaceae, Nymphaceae, and Cruciferae.Abstract:
Superoxide dismutases (EC 11511) in vascular plants representing different evolutionary levels were characterized using polyacrylamide gel electrophoresis The three forms of the enzyme were distinguished from each other based on the following criteria: a) the Cu-Zn enzyme is sensitive to cyanide wherease the Fe and Mn enzymes are not; and b) the Cu-Zn and Fe enzymes are inhibited by H2O2 whereas the Mn enzyme is H2O2-resistant Of the 43 plant families investigated, the Fe-containing superoxide dismutase was found in three families: Gingkoaceae, Nymphaceae, and Cruciferaeread more
Citations
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Journal ArticleDOI
Aspects of the structure, function, and applications of superoxide dismutase
TL;DR: After consideration of the question of superoxide toxicity and superoxide pathology, several areas in which SOD has been investigated or used as a tool in a biochemical, pharmacological, or clinical context are discussed.
BookDOI
Plant Salt Tolerance
Sergey Shabala,Tracey Ann Cuin +1 more
TL;DR: Preparing protoplasts and vacuoles to detect the functional expression of K + and cation channels of plasma membrane and tonoplast, as well as plasma membrane anion channels and vacUolar and plasma membrane H + pumps, which contribute to the intracellular ionic homeostasis under saline conditions are described.
Journal ArticleDOI
Superoxide Dismutase in Plants
TL;DR: In plants, three forms of the enzyme exist, as classified by their active site metal ion: copper/zinc, manganese, and iron forms, and it is only in plants that all three different types of SOD coexist.
Journal ArticleDOI
Toxic oxygen species and protective systems of the chloroplast
TL;DR: Salin et al. as discussed by the authors reviewed the production of oxyradicals and intermediates by chloroplasts as well as the means of protection are discussed in this review and concluded that these highly reactive intermediates pose the threat of toxicity unless neutralized by scavenger substrates or enzymes.
Journal ArticleDOI
Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.).
Bryan D. McKersie,Yurong Chen,M. De Beus,Steve Bowley,Chris Bowler,Dirk Inzé,K. D'halluin,J. Botterman +7 more
TL;DR: Detailed analysis of a set of four selected transformants indicated that some had enhanced SOD activity, increased tolerance to the diphenyl ether herbicide, acifluorfen, and increased regrowth after freezing stress, suggesting that Mn-SOD serves a protective role by minimizing oxygen free radical production after freezing Stress.
References
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Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more
TL;DR: The demonstration that O2·- can reduce ferricytochrome c and tetranitromethane, and that superoxide dismutase, by competing for the superoxide radicals, can markedly inhibit these reactions, is demonstrated.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.
Journal ArticleDOI
Superoxide Dismutases: I. Occurrence in Higher Plants
TL;DR: Nine of the enzyme activities were eliminated with cyanide treatment suggesting that they may be cupro-zinc enzymes, whereas one was cyanide-resistant and may be a manganese enzyme.
Journal ArticleDOI
Mitochondrial superoxide simutase. Site of synthesis and intramitochondrial localization.
TL;DR: This result indicates that the manganoenzyme, although localized in the mitochondrial matrix, is actually made under the directions of the nuclear genome and that its biosynthesis therefore probably takes place in the cytosol.
Journal ArticleDOI
Superoxide Dismutase from Escherichia coli B: A NEW MANGANESE-CONTAINING ENZYME
TL;DR: The bacterial superoxide dismutase, which catalyzes the disproportionation of univalently reduced oxygen, has now been purified from Escherichia coli and was found to contain manganese.
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Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more