Journal ArticleDOI
Down-regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA levels and synthesis in Syrian hamster C100 cells by the oxidosqualene cyclase inhibitor [4′-(6-allyl-ethyl-amino-hexyloxy)-2′-fluoro-phenyl]-(4-bromophenyl)-methanone (Ro 48–8071): comparison to simvastatin
TLDR
In vivo inhibition of 2,3-oxidosqualene:lanosterol cyclase by Ro 48-8071 results in an indirect down-regulation of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) activity, which is attributed to increased levels of oxysterols, produced upon partial inhibition of OSC, that suppress HMGR and other sterol-responsive genes.About:
This article is published in Biochemical Pharmacology.The article was published on 1998-08-15. It has received 15 citations till now. The article focuses on the topics: Cholesterol & Lanosterol synthase.read more
Citations
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Journal ArticleDOI
Regulation and deregulation of cholesterol homeostasis: The liver as a metabolic “power station”
TL;DR: An updated overview of liver cholesterol metabolism regulation and deregulation and the causes of cholesterol metabolism-related diseases is provided and current pharmacological treatment and novel hypocholesterolemic strategies will be introduced.
Journal ArticleDOI
Proteolysis and sterol regulation
TL;DR: This review describes the methods and results of various studies to understand the mechanisms and molecules involved in these distinct but interrelated aspects of sterol regulation and the intriguing similarities that appear to exist at the levels of protein sequence and cell biology.
Journal ArticleDOI
Dose-dependent effects of lovastatin on cell cycle progression. Distinct requirement of cholesterol and non-sterol mevalonate derivatives.
Javier Martínez-Botas,Antonio J. Ferruelo,Yajaira Suárez,Carlos Fernández,Diego Gómez-Coronado,Miguel A. Lasunción +5 more
TL;DR: The distinct roles of mevalonate, or its non-sterol derivatives, and cholesterol in cell cycle progression, both being required for normal cell cycling are demonstrated.
Journal ArticleDOI
An Oxysterol-derived Positive Signal for 3-Hydroxy- 3-methylglutaryl-CoA Reductase Degradation in Yeast
TL;DR: It is demonstrated, through the use of genetic and pharmacological manipulation of oxidosqualene-lanosterol cyclase, that an oxysterol-derived signal positively regulated HMGR degradation in yeast.
Journal ArticleDOI
Synthesis and structure-activity studies of novel orally active non-terpenoic 2,3-oxidosqualene cyclase inhibitors.
Henrietta Dehmlow,Johannes Aebi,Synese Jolidon,Yu-Hua Ji,Elisabeth von der Mark,Jacques Himber,Olivier Morand +6 more
TL;DR: The approach combined testing of the compounds first for increased binding affinity and for increased stability in vitro, and most promising compounds were then evaluated for their efficacy in lowering plasma total cholesterol (TC) and plasma low-density lipoprotein cholesterol (LDL-C) in hyperlipidemic hamsters.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
TL;DR: In this paper, a simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described, based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter.
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Regulation of the mevalonate pathway.
TL;DR: The mevalonate pathway produces isoprenoids that are vital for diverse cellular functions, ranging from cholesterol synthesis to growth control, and could be useful in treating certain forms of cancer as well as heart disease.
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Efficient trace-labelling of proteins with iodine.
TL;DR: Values greater than 50 per cent can be obtained by adding oxidizing agents to liberate iodine from iodide, but most if not all of these appear to affect adversely the properties of the labelled protein.
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The metabolism of very low density lipoprotein proteins. I. Preliminary in vitro and in vivo observations.
TL;DR: During the first 24 h after injection of 125I-labeled very low density lipoprotein to humans, the decay rate of low densitylipoprotein apoprotein from very lowdensity lipoproteins was faster than that of apolipoprotein glutamic acid, apoipoprotein alanine1 and apolipropoteinAlanine2.