Draft Genome Sequence of a Clinical Enterococcus faecium Sequence Type 18 Strain from South Africa.
Nontombi Marylucy Mbelle,Nontombi Marylucy Mbelle,Nontuthuko Excellent Maningi,Vhudzani Tshisevhe,Vhudzani Tshisevhe,Lesedi Modipane,Daniel G. Amoako,John Osei Sekyere +7 more
Reads0
Chats0
TLDR
The first draft genome sequence of an Enterococcus faecium sequence type 18 (ST18) strain isolated from a tuberculosis patient in Africa is reported.Abstract:
We report the first draft genome sequence of an Enterococcus faecium sequence type 18 (ST18) strain isolated from a tuberculosis patient in Africa. The genome is comprised of 3,202,539 bp, 501 contigs, 37.70% GC content, 3,202 protein-encoding genes, and 61 RNA genes. The resistome and virulome of this important pathogen are presented herein.read more
Citations
More filters
SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Journal ArticleDOI
The Resistome, Mobilome, Virulome and Phylogenomics of Multidrug-Resistant Escherichia coli Clinical Isolates from Pretoria, South Africa
Nontombi Marylucy Mbelle,Nontombi Marylucy Mbelle,Charles Feldman,John Osei Sekyere,Nontuthuko Excellent Maningi,Lesedi Modipane,Sabiha Y. Essack +6 more
TL;DR: A rich repertoire of virulence genes, including iss, gad and iha were identified, which is very worrying for clinical epidemiology and infectious diseases management in South Africa.
Journal ArticleDOI
Pathogenomics and Evolutionary Epidemiology of Multi-Drug Resistant Clinical Klebsiella pneumoniae Isolated from Pretoria, South Africa
Nontombi Marylucy Mbelle,Nontombi Marylucy Mbelle,Charles Feldman,John Osei Sekyere,Nontuthuko Excellent Maningi,Lesedi Modipane,Sabiha Y. Essack +6 more
TL;DR: The findings depict the rich resistome, mobilome and virulome repertoire in clinical K. pneumoniae strains, which are mainly transmitted by clonal, multiclonal and horizontal means in South Africa.
Journal ArticleDOI
Comparative genomics of vancomycin-resistant Enterococcus spp. revealed common resistome determinants from hospital wastewater to aquatic environments.
TL;DR: The VRE resistance quotient (RQ) of different environmental matrixes in wastewater is elucidated and genomic determinants of VRE strains recovered from HW to water resources are compared.
Journal ArticleDOI
Pathogenomic Analysis of a Novel Extensively Drug-Resistant Citrobacter freundii Isolate Carrying a blaNDM-1 Carbapenemase in South Africa.
Yogandree Ramsamy,Koleka Mlisana,Daniel G. Amoako,Mushal Allam,Arshad Ismail,Ravesh Singh,Akebe Luther King Abia,Sabiha Y. Essack +7 more
TL;DR: In this article, a novel carbapenem-resistant Citrobacter freundii isolate (H2730R) from a rectal swab of an adult male patient admitted to a tertiary hospital, Durban, South Africa was identified using selective media and API 20e kit.
References
More filters
Journal ArticleDOI
SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
Anton Bankevich,Sergey Nurk,Dmitry Antipov,Alexey Gurevich,Mikhail Dvorkin,Alexander S. Kulikov,Valery M. Lesin,Sergey I. Nikolenko,Son Pham,Andrey D. Prjibelski,Alexey V. Pyshkin,Alexander Sirotkin,Nikolay Vyahhi,Glenn Tesler,Max A. Alekseyev,Pavel A. Pevzner +15 more
TL;DR: SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies.
SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Journal ArticleDOI
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.
Todd M. Lowe,Sean R. Eddy +1 more
TL;DR: A program is described, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases.
Journal ArticleDOI
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Karin Lagesen,Peter Fischer Hallin,Einar Andreas Rødland,Hans Henrik Stærfeldt,Torbjørn Rognes,David W. Ussery +5 more
TL;DR: Results from running RNAmmer on a large set of genomes indicate that the location of rRNAs can be predicted with a very high level of accuracy.
Journal ArticleDOI
Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
TL;DR: A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.