RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Karin Lagesen,Peter Fischer Hallin,Einar Andreas Rødland,Hans Henrik Stærfeldt,Torbjørn Rognes,David W. Ussery +5 more
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TLDR
Results from running RNAmmer on a large set of genomes indicate that the location of rRNAs can be predicted with a very high level of accuracy.Abstract:
The publication of a complete genome sequence is usually accompanied by annotations of its genes. In contrast to protein coding genes, genes for ribosomal RNA (rRNA) are often poorly or inconsistently annotated. This makes comparative studies based on rRNA genes difficult. We have therefore created computational predictors for the major rRNA species from all kingdoms of life and compiled them into a program called RNAmmer. The program uses hidden Markov models trained on data from the 5S ribosomal RNA database and the European ribosomal RNA database project. A pre-screening step makes the method fast with little loss of sensitivity, enabling the analysis of a complete bacterial genome in less than a minute. Results from running RNAmmer on a large set of genomes indicate that the location of rRNAs can be predicted with a very high level of accuracy. Novel, unannotated rRNAs are also predicted in many genomes. The software as well as the genome analysis results are available at the CBS web server.read more
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The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
Christian Quast,Elmar Pruesse,Pelin Yilmaz,Jan Gerken,Timmy Schweer,Pablo Yarza,Jörg Peplies,Frank Oliver Glöckner +7 more
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Prokka: Rapid Prokaryotic Genome Annotation
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The Ribosomal Database Project: improved alignments and new tools for rRNA analysis
James R. Cole,Qiong Wang,Erick Cardenas,Jordan A. Fish,Benli Chai,Ryan J. Farris,A. S. Kulam-Syed-Mohideen,Donna M. McGarrell,Terry L. Marsh,George M. Garrity,James M. Tiedje +10 more
TL;DR: An improved alignment strategy uses the Infernal secondary structure aware aligner to provide a more consistent higher quality alignment and faster processing of user sequences, and a new Pyrosequencing Pipeline that provides tools to support analysis of ultra high-throughput rRNA sequencing data.
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Gut Microbiota from Twins Discordant for Obesity Modulate Metabolism in Mice
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Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
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TL;DR: A new chimera detection tool called Chimera Slayer (CS), which detects chimeras with greater sensitivity than previous methods, performs well on short sequences such as those produced by the 454 Life Sciences (Roche) Genome Sequencer, and can scale to large data sets.
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