Journal ArticleDOI
Dynamic instability of microtubule growth
TLDR
It is reported here that microtubules in vitro coexist in growing and shrinking populations which interconvert rather infrequently and this dynamic instability is a general property of micro Tubules and may be fundamental in explaining cellular microtubule organization.Abstract:
We report here that microtubules in vitro coexist in growing and shrinking populations which interconvert rather infrequently. This dynamic instability is a general property of microtubules and may be fundamental in explaining cellular microtubule organization.read more
Citations
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Journal ArticleDOI
Microtubules as a target for anticancer drugs.
Mary Ann Jordan,Leslie Wilson +1 more
TL;DR: Highly dynamic mitotic-spindle microtubules are among the most successful targets for anticancer therapy, and it is now known that at lower concentrations, microtubule-targeted drugs can suppress micro Tubule dynamics without changingmicrotubule mass; this action leads to mitotic block and apoptosis.
Journal ArticleDOI
Ischemic Cell Death in Brain Neurons
TL;DR: A major unifying thread of the review is a consideration of how the changes occurring during and after ischemia conspire to produce damaging levels of free radicals and peroxynitrite to activate calpain and other Ca(2+)-driven processes that are damaging, and to initiate the apoptotic process.
Journal ArticleDOI
Microtubule polymerization dynamics
TL;DR: This review describes progress toward understanding the mechanism of dynamic instability of pure tubulin and discusses the function and regulation of microtubule dynamic instability in living cells.
Journal ArticleDOI
Cell mechanics and the cytoskeleton
TL;DR: An important insight emerging from this work is that long-lived cytoskeletal structures may act as epigenetic determinants of cell shape, function and fate.
Journal ArticleDOI
Small Molecule Inhibitor of Mitotic Spindle Bipolarity Identified in a Phenotype-Based Screen
Thomas U. Mayer,Tarun M. Kapoor,Stephen J. Haggarty,Stephen J. Haggarty,Randall W. King,Stuart L. Schreiber,Stuart L. Schreiber,Timothy J. Mitchison +7 more
TL;DR: In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity, and will therefore be a particularly useful tool for studying mitotic mechanisms.
References
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Journal ArticleDOI
Microtubule assembly nucleated by isolated centrosomes
TL;DR: It is reported here that purified centrosomes nucleate the assembly of microtubules with unusual dynamic properties, which may have important implications for the mechanism by which microtubule arrays are organized and stabilized in cells.
Journal ArticleDOI
Opposite end assembly and disassembly of microtubules at steady state in vitro
Robert L. Margolis,Leslie Wilson +1 more
TL;DR: It is concluded that the microtubule assembly-disassembly "equilibrium" is a steady state summation of two different reactions which occur at opposite ends of the micro Tubule, and that assembly and disassembly occur predominantly and perhaps exclusively at the opposite ends under steady state conditions in vitro.
Journal ArticleDOI
Head-to-tail polymerization of microtubules in vitro. Electron microscope analysis of seeded assembly.
TL;DR: The plus end of the microtubule is identified as its head and "s," the head-to-tail polymerization parameter is calculated, Surprisingly small values (s = 0.07 +/- 0.02) were found.
Journal ArticleDOI
Kinetic analysis of guanosine 5'-triphosphate hydrolysis associated with tubulin polymerization
M F Carlier,D Pantaloni +1 more
TL;DR: The possibility is discussed that after assembly tubulin undergoes a conformation change which could trigger GTP hydrolysis and sequestration of GDP.