Journal ArticleDOI
Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei)
Reads0
Chats0
TLDR
The results indicated that the probiotic B. subtilis could significantly promote the growth rate of shrimp by increasing the immune function and antioxidant capacity.Abstract:
The objective of this study was to evaluate the effect of dietary supplementation of a probiotic bacterium, Bacillus subtilis, on the growth, immune response and antioxidant activities of shrimp (Litopenaeus vannamei). Shrimps with an average initial weight of 2.11±0.17 g were randomly assigned to four groups with three replicates. The control group was fed a basal diet, and three treated groups were fed diets supplemented with B. subtilis at doses of 1 × 104, 5 × 104 and 10 × 104 colony-forming unit (CFU) g−1 feed respectively. After 40 days of culture, 10 shrimps from each replicate were taken randomly for the determination of immune response and oxidization resistance indices. The results showed that the shrimps fed with B. subtilis at a dose of 1 × 104, 5 × 104 CFU g−1 feed showed significantly better growth than that of the control diet. The phenoloxidase activities showed a tendency to increase with an increased dose of B. subtilis in diets but there was no significant difference among the three treated groups. In addition, phenoloxidase activities were found to be significantly higher (P<0.05) in the groups treated with B. subtilis than that of the control group. Shrimps treated with 5 × 104 CFU g−1 feed probiotic bacterium showed the highest lysozyme activity and it was significantly higher (P<0.05) than the other groups. However, there was no significant difference in acid phosphatase and alkaline phosphatase activity across all the groups. The total antioxidant capacity, superoxide dismutase and glutathione peroxidase activities in the probiotic-treated groups were significantly increased (P<0.05) as compared with the control groups. Both maleic dialdehyde concentration and superoxide anion activities in the probiotic-treated groups were significantly lower (P<0.05) than those of the control. The probiotic did not affect the nitric oxide synthase and the catalase activity in any of the control and treated groups. These results indicated that the probiotic B. subtilis could significantly promote the growth rate of shrimp by increasing the immune function and antioxidant capacity. The most effective dose of B. subtilis in the diet was 5 × 104 CFU g−1 feed.read more
Citations
More filters
Journal ArticleDOI
Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei.
Hadi Zokaeifar,José Luis Balcázar,Che Roos Saad,Mohd Salleh Kamarudin,Kamaruzaman Sijam,Aziz Arshad,Naghmeh Nejat +6 more
TL;DR: It is demonstrated that administration of B. subtilis strains, L10 and G1, can improve growth performance and disease resistance through an enhanced immune response in shrimp.
Journal ArticleDOI
Use of probiotics in aquaculture of China-a review of the past decade.
Anran Wang,Chao Ran,Yanbo Wang,Zhen Zhang,Qianwen Ding,Yalin Yang,Rolf Erik Olsen,Einar Ringø,Jérôme Bindelle,Zhigang Zhou +9 more
TL;DR: Wang et al. as mentioned in this paper discussed species, effects, and mode of actions of probiotics in Chinese aquaculture since 2008, and the challenges and future directions for research are also discussed.
Journal ArticleDOI
Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses.
Bidhan Chandra De,Dharmendra Kumar Meena,Bijay Kumar Behera,Pronob Das,P. K. Das Mohapatra,A. P. Sharma +5 more
TL;DR: This review highlights the classifications and applications of probiotics in Aquaculture, and summarizes the advancement and research highlights of the probiotic status and mode of action, which are of great significance from an ecofriendly, sustainable, intensive aquaculture point of view.
Journal ArticleDOI
Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis).
TL;DR: Dietary FOS and B. licheniformis could significantly enhance the innate immunity and antioxidant capability of triangular bream, as well as improve its disease resistance.
Journal ArticleDOI
Effects of potential probiotic Bacillus subtilis T13 on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumber Apostichopus japonicus.
TL;DR: The present study confirmed the potential beneficial effects of B. subtilis T13 as dietary probiotic in juvenile A. japonicus and confirmed the cumulative mortality after V. splendidus challenge decreased significantly in sea cucumbers fed with T13 at dose of 10(9) CFU/g.
References
More filters
Journal ArticleDOI
The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power' : the FRAP assay
TL;DR: The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
Journal ArticleDOI
Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more
TL;DR: The demonstration that O2·- can reduce ferricytochrome c and tetranitromethane, and that superoxide dismutase, by competing for the superoxide radicals, can markedly inhibit these reactions, is demonstrated.
Book ChapterDOI
Microsomal lipid peroxidation.
John A. Buege,Steven D. Aust +1 more
TL;DR: This chapter discusses microsomal lipid peroxidation, a complex process known to occur in both plants and animals that involves the formation and propagation of lipid radicals, the uptake of oxygen, a rearrangement of the double bonds in unsaturated lipids, and the eventual destruction of membrane lipids.
Journal ArticleDOI
Glutathione peroxidase activity in selenium-deficient rat liver☆
TL;DR: Two peaks of glutathione peroxidase activity were present in the Sephadex G-150 gel filtration chromatogram of rat liver supernatant when 1.5 mM cumene hydroperoxide was used as substrate, and the second peak represents a second glutathienase activity which catalyzes the destruction of organic hydroperoxides but has little activity toward H 2 O 2 and which persists in severe selenium deficiency.
Journal ArticleDOI
Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification.
Ron Kohen,Abraham Nyska +1 more
TL;DR: This review includes different topics essential for understanding oxidative stress phenomena and provides tools for those intending to conduct study and research in this field.