Journal ArticleDOI
Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain
TLDR
In this paper, the effect of hemicellulose and lignin on cellulose conversion into glucose was investigated using a commercial cellulase concentrate (Celluclast 1.5L) in an enzyme/substrate ratio of 45 FPU/g, 2% (w/v) substrate concentration, 45°C for 96h.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2008-08-05. It has received 315 citations till now. The article focuses on the topics: Cellulose & Hemicellulose.read more
Citations
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A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes--factors affecting enzymes, conversion and synergy.
J.S. Van Dyk,Brett I. Pletschke +1 more
TL;DR: This review examines the enzymes required to degrade various components of lignocellulose and the impact of pretreatments on the lignosic substrates and the enzyme required for degradation and the effect of and interaction between different hemicellulases on complex substrates.
Journal ArticleDOI
Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose
TL;DR: In this article, the effects of chemical compositions and physical structures on the enzymatic digestibility of lignocellulosic biomass were summarized and the authors suggest that future work should be focused on but not limited to the molecular mechanisms of biomass recalcitrance by investigating the micro-scale and nanoscale features as well as hydrogen bonds network of LBSs.
Journal ArticleDOI
The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials.
TL;DR: The combined pretreatment strategies are reviewed for improving the enzymatic hydrolysis of lignocellulose and realizing the comprehensive utilization of lignecellulosic materials.
The role of pretreatment in improving the enzymatic hydrolysis
TL;DR: In this article, the chemical structure of lignocellulosic biomass and factors likely affect the digestibility of LCLs are discussed, and then an overview about the most important pretreatment processes available are provided.
Journal ArticleDOI
Facile fractionation of lignocelluloses by biomass-derived deep eutectic solvent (DES) pretreatment for cellulose enzymatic hydrolysis and lignin valorization
Xiaojun Shen,Xiaojun Shen,Jia-Long Wen,Qingqing Mei,Xue Chen,Dan Sun,Tong-Qi Yuan,Run-Cang Sun +7 more
TL;DR: In this paper, a biomass-derived deep eutectic solvent (DES) pretreatment was developed to deconstruct the recalcitrant structure of Eucalyptus for further cellulose enzymatic hydrolysis and lignin valorization.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
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Hydrolysis of lignocellulosic materials for ethanol production: a review.
Ye Sun,Jiayang Cheng +1 more
TL;DR: Simultaneous saccharification and fermentation effectively removes glucose, which is an inhibitor to cellulase activity, thus increasing the yield and rate of cellulose hydrolysis, thereby increasing the cost of ethanol production from lignocellulosic materials.
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The biological degradation of cellulose
Pierre Béguin,Jean-Paul Aubert +1 more
TL;DR: The study of cellulolytic enzymes at the molecular level has revealed some of the features that contribute to their activity and an increasing number of three-dimensional structures are becoming available for cellulases and xylanases belonging to different families, which will provide paradigms for molecular modeling of related enzymes.
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Fundamental factors affecting biomass enzymatic reactivity.
TL;DR: An empirical model was identified that describes the roles of lignin content, acetyl contents, and crystallinity indices in enzymatic hydrolysis and the digestibility of several lime-treated biomass samples agreed with the empirical model.