Journal ArticleDOI
Effect of treatment protocol and sample time on the frequencies of micronucleated polychromatic erythrocytes in mouse bone marrow and peripheral blood
TLDR
A comparison of the frequency of micronucleated PCE in peripheral blood and bone marrow following the treatment of mice with either BZD or DMBA suggests that, following a three injection protocol, either tissue can be used with equal efficacy.Abstract:
Studies were conducted to evaluate the effect of experimental protocol on the ability of benzidine (BZD), dimethylbenzanthracene (DMBA) and mitomycin C (MMC), administered by intraperitoneal injection, to induce micronuclei in polychromatic erythrocytes (PCE) of B6C3F1 mice. Three different treatment/sampling protocols were used, involving from one to three consecutive daily treatments and from three to one, respectively, consecutive daily samplings beginning 24 h after the last injection. DMBA and MMC elicited a significant micronucleus response in all three experimental protocols, while BZD induced a significant response only in the multiple injection protocols. Of the three protocols, the 3-day injection/single sample time protocol offers the greatest efficiency in minimizing the number of animals required in a study, in decreasing the time needed for scoring and in simplifying the statistical analysis. In addition, a comparison of the frequency of micronucleated PCE in peripheral blood and bone marrow following the treatment of mice with either BZD or DMBA suggests that, following a three injection protocol, either tissue can be used with equal efficacy.read more
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Evaluation of a three-exposure mouse bone marrow micronucleus protocol: Results with 49 chemicals
TL;DR: This study was conducted to assess the ability of the micronucleus test to discriminate between rodent carcinogens and noncarcinogens and to determine its potential role, in combination with other short‐term tests, in identifying genotoxic chemicals that present a carcinogenic hazard.
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Effect of hepatic methyl donor status on urinary excretion and DNA damage in B6C3F1 mice treated with sodium arsenite.
TL;DR: Results indicate that hepatic methyl donors deficiency significantly decreases the total urinary excretion of orally administered sodium arsenite and markedly modulates target organ arsenic-induced DNA damage, with an apparent shift from liver and bladder to skin.
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Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining : The summary report of the 5th collaborative study by CSGMT/JEMS MMS
TL;DR: The results suggest that the peripheral blood assay using the AO supravital staining technique generates reproducible and reliable data to evaluate the clastogenicity of chemicals, which makes the peripheralBlood micronucleus assay an attractive alternative to the conventional bone marrow assay.
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Genotoxicity biomonitoring in coal regions using wild rodent Ctenomys torquatus by Comet assay and micronucleus test.
Juliana da Silva,Thales Renato Ochotorena de Freitas,Vanina D. Heuser,Jorge Reppold Marinho,Bernardo Erdtmann +4 more
TL;DR: The Comet assay was more sensitive and showed a direct relationship between age and damage, and an inverse relationship between temperature and damage index, and the results showed that coal and derivatives induced DNA and chromosomal lesions in rodent cells that were demonstrated by Comet and MN assays.
Journal ArticleDOI
Bone marrow micronucleus assay: a review of the mouse stocks used and their published mean spontaneous micronucleus frequencies.
TL;DR: This analysis bears out the recommendation of the first Gene‐Tox Report on the micronucleus assay that the historical negative control frequency for a mouse stock should fall between 1 and 3 mnPCE/1,000 and suggests that the recommended range for historical mean frequency should be extended slightly.