Journal ArticleDOI
Guidelines for the conduct of micronucleus assays in mammalian bone marrow erythrocytes.
James T. MacGregor,John A. Heddle,Mark Hite,Barry H. Margolin,Clacs Ramel,Michael F. Salamone,Raymond R. Tice,Dieter Wild +7 more
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This article is published in Mutation Research\/genetic Toxicology.The article was published on 1987-10-01. It has received 352 citations till now. The article focuses on the topics: Micronucleus test & Micronucleus.read more
Citations
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Journal ArticleDOI
Twenty-Eight-Day Oral Toxicity, Genotoxicity, and Gender-Related Tissue Distribution of Silver Nanoparticles in Sprague-Dawley Rats
Yong Soon Kim,Jin Sik Kim,Hyun Sun Cho,Dae Sik Rha,Jae Min Kim,Jung Duck Park,Byung-Sun Choi,Ruth Lim,Hee Kyung Chang,Yong Hyun Chung,Il Hoon Kwon,Jayoung Jeong,Beom Seok Han,Il Je Yu +13 more
TL;DR: The present results suggest that silver nanoparticles do not induce genetic toxicity in male and female rat bone marrow in vivo, and the tissue distribution of silver nanopaticles did show a dose-dependent accumulation of silver content in all the tissues examined.
Journal ArticleDOI
Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies
A.B Richards,S Krakowka,L.B Dexter,H Schmid,A.P.M Wolterbeek,D.H Waalkens-Berendsen,A Shigoyuki,M Kurimoto +7 more
TL;DR: It is concluded that trehalose is safe for use as an ingredient in consumer products when used in accordance with current Good Manufacturing Practices.
Journal ArticleDOI
The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gene-Tox Program
TL;DR: The protocol recommended for the micronucleus assay in mammalian bone marrow has been revised and simplified and the minimum number of cells to be scored per treatment group has been increased to 20,000 to increase the ability of the assay to detect a doubling of the control micron nucleus frequency.
Journal ArticleDOI
Proteomic Approach to Understanding Antibiotic Action
TL;DR: Proteomic technology is used to elucidate the complex cellular responses of Bacillus subtilis to antimicrobial compounds belonging to classical and emerging antibiotic classes and suggests that novel compounds with unknown mechanisms of action may be classified.
Journal ArticleDOI
In vivo rodent erythrocyte micronucleus assay.
M. Hayashi,Raymond R. Tice,James T. MacGregor,Diana Anderson,David H. Blakey,M. Kirsh-Volders,Frederick F.B. Oleson,Francesca Pacchierotti,Felix F. Romagna,Hiroyasu Shimada,Sizuyo Sutou,B. Vannier +11 more
TL;DR: Although a consensus was not achieved, operationally it was agreed that 2000 cells per animal and four animals per group was a minimum requirement, in general, the available database suggests that the use of one gender is adequate for screening.
References
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Journal ArticleDOI
The induction of micronuclei as a measure of genotoxicity: A report of the U.S. environmental protection agency Gene-Tox program☆
John A. Heddle,Mark Hite,Barbara Kirkhart,Kathleen H. Mavournin,James T. MacGregor,Gordon W. Newell,Michael F. Salamone +6 more
TL;DR: The most important recommendations in this report are: (1) at least 500 PCE should be examined from each of 8 animals to detect an increase of about 4‰ (per thousand) PCE when the background is less than 4 per 1000, and (2) the highest possible doses should be used.
Book ChapterDOI
The Micronucleus Test for Cytogenetic Analysis
TL;DR: Micronuclei originate from chromatin which for different reasons has been lagging in anaphase, and in the course of telophase this material is included into one or the other daughter cell where it either can fuse with the main nucleus or form one or several secondary nuclei.
Journal ArticleDOI
An application of Acridine Orange fluorescent staining to the micronucleus test.
TL;DR: In rat bone-marrow cells, micronuclei with green fluorescence could be easily distinguished from granules accidentally dispersed from broken mast cells, which showed bright red fluorescence, so it is recommended that the Acridine Orange staining method be used to provide more reliable data in the micron nucleus test.
Journal ArticleDOI
Statistical analyses for in vitro cytogenetic assays using Chinese hamster ovary cells
B. H. Margolin,Michael A. Resnick,J. Y. Rimpo,P. Archer,Sheila M. Galloway,A. D. Bloom,Errol Zeiger +6 more
TL;DR: In this paper, a trend test for evidence of a dose response is proposed for such SCE data, where the percent of cells with chromosome aberrations is the response of interest, and Monte Carlo methods are used to show that the trend test is more sensitive than four other statistical procedures considered for the analysis of Poisson-distributed SCE.