Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å.
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TLDR
A user-friendly, computationally-efficient 3D-CTF correction tool, NovaCTF, that is compatible with standard tomogram reconstruction workflows in IMOD is presented and it is found that high-resolution structures can be obtained with much smaller subtomogram averaging datasets than are required using 2D- CTF.About:
This article is published in Journal of Structural Biology.The article was published on 2017-09-01 and is currently open access. It has received 198 citations till now.read more
Citations
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Determining structures in a native environment using single-particle cryoelectron microscopy images.
TL;DR: In this paper, the densities of non-target proteins are treated as non-Gaussian noise, and a new target function is developed to improve the efficiency of recognizing the target protein in a single image.
Journal ArticleDOI
Efficient manual annotation of cryogenic electron tomograms using IMOD
TL;DR: In this paper , the authors present a manual annotation protocol using the open-source software IMOD and describe segmentation of three types of common cellular features: membranes, large globules, and filaments.
Journal ArticleDOI
Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
TL;DR: In this article , the authors performed electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of regular cylindrical morphology.
Posted ContentDOI
Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM
TL;DR: In this article , the authors used a combination of lattice templating and single particle analysis (SPA) to determine high-resolution structures of continuous regions of the HIV-1 capsid lattice containing both hexamers and pentamers.
Journal ArticleDOI
A feature-guided, focused 3D signal permutation method for subtomogram averaging
TL;DR: Maintenance of feature-guided alignment and 3D signal permutation leads to improved subtomogram average maps for a dataset of synaptic protein complexes in their native environment.
References
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Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
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Alexis Rohou,Nikolaus Grigorieff +1 more
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Book
Extrapolation, Interpolation, and Smoothing of Stationary Time Series: With Engineering Applications
TL;DR: Extrapolation interpolation and smoothing of stationary, stationary tones interference cancellation using adaptive and stationary time series financial definition of stationary.