Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å.
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TLDR
A user-friendly, computationally-efficient 3D-CTF correction tool, NovaCTF, that is compatible with standard tomogram reconstruction workflows in IMOD is presented and it is found that high-resolution structures can be obtained with much smaller subtomogram averaging datasets than are required using 2D- CTF.About:
This article is published in Journal of Structural Biology.The article was published on 2017-09-01 and is currently open access. It has received 198 citations till now.read more
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Journal ArticleDOI
Advances in cryo-electron tomography for biology and medicine.
TL;DR: In this follow-up, this basic description of CET is extended in order to explain the aforementioned recent advances, and describe related 3D techniques that can be applied to the anatomy of biological systems that are relevant for medicine.
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Cone-shaped HIV-1 capsids are transported through intact nuclear pores
Vojtech Zila,Erica Margiotta,Beata Turonova,Thorsten G. Müller,Christian E. Zimmerli,Simone Mattei,Simone Mattei,Matteo Allegretti,Kathleen Börner,Jona Rada,Barbara Müller,Marina Lusic,Hans-Georg Kräusslich,Martin Beck,Martin Beck +14 more
TL;DR: It is demonstrated that the diameter of the NPC in cellulo is sufficient for the import of apparently intact, coneshaped capsids and detected disrupted and empty capsid fragments, indicating that uncoating of the replication complex occurs by breaking the capsid open, and not by disassembly into individual subunits.
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In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer.
Andriko von Kügelgen,Haiping Tang,Gail G. Hardy,Danguole Kureisaite-Ciziene,Yves V. Brun,Yves V. Brun,Phillip J. Stansfeld,Carol V. Robinson,Tanmay A.M. Bharat +8 more
TL;DR: A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.
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Microtubule architecture in vitro and in cells revealed by cryo-electron tomography
Joseph Atherton,Melissa A. Stouffer,Melissa A. Stouffer,Fiona Francis,Fiona Francis,Carolyn A. Moores +5 more
TL;DR: The results of cryo-electron tomography experiments on in vitro-polymerized microtubules and comparisons with microtubule ultrastructure in cells are described.
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Advances in cryo-electron tomography and subtomogram averaging and classification.
TL;DR: Cryo-electron tomography (cryoET) subtomogram averaging has emerged as a structural biology method for sparse and heterogenerous sampls and can delineate different conformational states of macromolecular complexes.
References
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Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
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