Journal ArticleDOI
Computer Visualization of Three-Dimensional Image Data Using IMOD
TLDR
IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.About:
This article is published in Journal of Structural Biology.The article was published on 1996-01-01. It has received 4830 citations till now. The article focuses on the topics: Visualization & Computer graphics.read more
Citations
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Automated electron microscope tomography using robust prediction of specimen movements.
TL;DR: A new method was developed to acquire images automatically at a series of specimen tilts, as required for tomographic reconstruction, using changes in specimen position at previous tilt angles to predict the position at the current tilt angle.
Journal ArticleDOI
EMAN2: an extensible image processing suite for electron microscopy.
Guang Tang,Liwei Peng,Philip R. Baldwin,Deepinder S. Mann,Wen Jiang,I. Rees,Steven J. Ludtke +6 more
TL;DR: EMAN2 has been under development for the last two years, with a completely refactored image processing library, and a wide range of features to make it much more flexible and extensible than EMAN1.
Journal ArticleDOI
ER Tubules Mark Sites of Mitochondrial Division
Jonathan R. Friedman,Laura L. Lackner,Matthew West,Jared R. DiBenedetto,Jodi Nunnari,Gia K. Voeltz +5 more
TL;DR: It was found that mitochondrial division occurred at positions where ER tubules contacted mitochondria and mediated constriction before Drp1 recruitment, suggesting that ERtubules may play an active role in defining the position of mitochondrial division sites.
Journal ArticleDOI
Dual-axis tomography: an approach with alignment methods that preserve resolution.
TL;DR: Analysis by Fourier sector correlation indicated that the variable tilt increment improved the reconstruction in some respects but degraded it in others, and a varying tilt increment thus does not give an unqualified improvement, at least when using back-projection algorithms for the reconstruction.
Journal ArticleDOI
A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
Mitsuko Hayashi-Nishino,Naonobu Fujita,Takeshi Noda,Akihito Yamaguchi,Tamotsu Yoshimori,Akitsugu Yamamoto +5 more
TL;DR: Electron tomography revealed that the ER–IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.
References
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Journal ArticleDOI
The Reconstruction of a Three-Dimensional Structure from Projections and its Application to Electron Microscopy
TL;DR: In this paper, the Fourier transform is used to combine data from a number of different views of a transmission electron micrograph to reconstruct a 3D image of a given particle to a given degree of resolution.
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The nematode Caenorhabditis elegans
TL;DR: In Caenorhabditis elegans patterns of cell division, differentiation, and morphogenesis can be observed with single-cell resolution in intact, living animals.
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Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle.
Mark Winey,C L Mamay,Eileen T. O'Toole,David N. Mastronarde,Thomas H. Giddings,K L McDonald,J R McIntosh +6 more
TL;DR: The three dimensional organization of microtubules in mitotic spindles of the yeast Saccharomyces cerevisiae has been determined by computer- aided reconstruction from electron micrographs of serially cross- sectioned spindes, and interpretation of these reconstructed spindle offers some insights into the mechanisms of mitosis in this yeast.
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A new method for image segmentation
TL;DR: A new method for image segmentation via adaptive thresholding is presented, where the threshold surface is determined by interpolating the image gray levels at points where the gradient is high, indicating probable object edges.
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Interpolar spindle microtubules in PTK cells.
TL;DR: Spindle microtubules in PtK1 cells, fixed at stages from metaphase to telophase, have been reconstructed using serial sections, electron microscopy, and computer image processing to study the class ofMTs that form an interdigitating system connecting the two spindle poles and their relationship to the spindle MTs that attach to kinetochores.