Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å.
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TLDR
A user-friendly, computationally-efficient 3D-CTF correction tool, NovaCTF, that is compatible with standard tomogram reconstruction workflows in IMOD is presented and it is found that high-resolution structures can be obtained with much smaller subtomogram averaging datasets than are required using 2D- CTF.About:
This article is published in Journal of Structural Biology.The article was published on 2017-09-01 and is currently open access. It has received 198 citations till now.read more
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In-cell NMR: Why and how?
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Book ChapterDOI
In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography.
Mohammed Kaplan,William J Nicolas,Wei Zhao,Stephen D. Carter,Lauren Ann Metskas,Georges Chreifi,Debnath Ghosal,Debnath Ghosal,Grant J. Jensen,Grant J. Jensen +9 more
TL;DR: In this paper, the authors present an outline of how to perform electron-cryo-tomography studies on a wide variety of biological samples including prokaryotic and eukaryotic cells and biological plant tissues.
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Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation
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Posted ContentDOI
Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography
Joseph Atherton,Joseph Atherton,Melissa A. Stouffer,Fiona Francis,Fiona Francis,Carolyn A. Moores +5 more
TL;DR: In this paper, a detailed analysis of the 3D structure of the growth cone of a mouse hippocampal neuron was performed using a cryo-electron tomography (CET) to directly visualize their three-dimensional subcellular architecture.
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The development of cryo-EM and how it has advanced microbiology
TL;DR: The 2017 Nobel Prize in Chemistry was awarded to Jacques Dubochet, Richard Henderson and Joachim Frank for the development of cryo-electron microscopy, a technique for high-resolution structural determination of biomolecules in solution that has provided unprecedented insight into the biology of microorganisms.
References
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Journal ArticleDOI
Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
Journal ArticleDOI
CTFFIND4: Fast and accurate defocus estimation from electron micrographs.
Alexis Rohou,Nikolaus Grigorieff +1 more
TL;DR: Modifications to the CTFFIND algorithm are described which make it significantly faster and more suitable for use with images collected using modern technologies such as dose fractionation and phase plates.
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Simultaneous algebraic reconstruction technique (SART): a superior implementation of the art algorithm.
TL;DR: This implementation of the Algebraic Reconstruction Technique appears to have a computational advantage over the more traditional implementation of ART and potential applications include image reconstruction in conjunction with ray tracing for ultrasound and microwave tomography.
Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
TL;DR: Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.
Book
Extrapolation, Interpolation, and Smoothing of Stationary Time Series: With Engineering Applications
TL;DR: Extrapolation interpolation and smoothing of stationary, stationary tones interference cancellation using adaptive and stationary time series financial definition of stationary.