Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging.
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TLDR
A “dose-symmetric tilt-scheme” that begins at low tilt and then alternates between increasingly positive and negative tilts maximizes the amount of high-resolution information maintained in the tomogram for subsequent subtomogram averaging, and may also be advantageous for other applications.About:
This article is published in Journal of Structural Biology.The article was published on 2017-02-01 and is currently open access. It has received 489 citations till now. The article focuses on the topics: Electron tomography.read more
Citations
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Journal ArticleDOI
Structures and distributions of SARS-CoV-2 spike proteins on intact virions.
Zunlong Ke,Joaquín Otón,Kun Qu,Mirko Cortese,Vojtech Zila,Lesley McKeane,Takanori Nakane,Jasenko Zivanov,Christopher J. Neufeldt,Berati Cerikan,John M. Lu,Julia Peukes,Xiaoli Xiong,Hans-Georg Kräusslich,Sjors H.W. Scheres,Ralf Bartenschlager,Ralf Bartenschlager,John A. G. Briggs +17 more
TL;DR: Cryo-electron microscopy and tomography is applied to image intact SARS-CoV-2 virions, determining the high-resolution structure, conformational flexibility and distribution of S trimers in situ on the virion surface and providing a basis from which to understand interactions between S and neutralizing antibodies during infection or vaccination.
Journal ArticleDOI
Real-time cryo-electron microscopy data preprocessing with Warp.
Dimitry Tegunov,Patrick Cramer +1 more
TL;DR: Warp is described, a software that automates all preprocessing steps of cryo-EM data acquisition and enables real-time evaluation, and includes deep-learning-based models for accurate particle picking and image denoising.
Journal ArticleDOI
Molecular Architecture of the SARS-CoV-2 Virus
Hangping Yao,Yutong Song,Yong Chen,Nanping Wu,Jialu Xu,Chujie Sun,Jiaxing Zhang,Tianhao Weng,Zheyuan Zhang,Zhigang Wu,Linfang Cheng,Danrong Shi,Xiangyun Lu,Jianlin Lei,Max Crispin,Yigong Shi,Lanjuan Li,Sai Li +17 more
TL;DR: Overall, these characterizations have revealed the architecture of the SARS-CoV-2 virus in exceptional detail, and shed lights on how the virus packs its ∼30 kb long single-segmented RNA in the ∼80 nm diameter lumen.
Journal ArticleDOI
Lewy pathology in Parkinson's disease consists of crowded organelles and lipid membranes.
Sarah H Shahmoradian,Sarah H Shahmoradian,Amanda J Lewis,Christel Genoud,Jürgen Hench,Tim Moors,Paula P Navarro,Daniel Castaño-Díez,Gabriel Schweighauser,Alexandra Graff-Meyer,Kenneth N. Goldie,Rosmarie Sütterlin,Evelien Huisman,Angela Ingrassia,Yvonne de Gier,Annemieke J.M. Rozemuller,Jing Wang,Anne De Paepe,Johannes Erny,Andreas Staempfli,Joerg Hoernschemeyer,Frederik Großerüschkamp,Daniel Niedieker,Samir F. El-Mashtoly,Marialuisa Quadri,Wilfred F. J. van IJcken,Vincenzo Bonifati,Klaus Gerwert,Bernd Bohrmann,Stephan Frank,Markus Britschgi,Henning Stahlberg,Wilma D.J. van de Berg,Matthias E. Lauer +33 more
TL;DR: Using three-dimensional correlative light and electron microscopy of Lewy bodies and Lewy neurites in postmortem brains of Parkinson’s disease patients, researchers show that the major constituents are membranes rather than proteinaceous filaments.
Journal ArticleDOI
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation
Jordina Guillén-Boixet,Andrii Kopach,Alex S. Holehouse,Sina Wittmann,Marcus Jahnel,Marcus Jahnel,Raimund Schlüßler,Kyoohyun Kim,Irmela R.E.A. Trussina,Jie Wang,Daniel Mateju,Ina Poser,Shovamayee Maharana,Martine Ruer-Gruß,Doris Richter,Xiaojie Zhang,Young-Tae Chang,Jochen Guck,Alf Honigmann,Julia Mahamid,Anthony A. Hyman,Rohit V. Pappu,Simon Alberti,Simon Alberti,Titus M. Franzmann +24 more
TL;DR: It is proposed that condensation coupled to conformational rearrangements and heterotypic multivalent interactions may be a general principle underlying RNP granule assembly.
References
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Journal ArticleDOI
Automated electron microscope tomography using robust prediction of specimen movements.
TL;DR: A new method was developed to acquire images automatically at a series of specimen tilts, as required for tomographic reconstruction, using changes in specimen position at previous tilt angles to predict the position at the current tilt angle.
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Dual-axis tomography: an approach with alignment methods that preserve resolution.
TL;DR: Analysis by Fourier sector correlation indicated that the variable tilt increment improved the reconstruction in some respects but degraded it in others, and a varying tilt increment thus does not give an unqualified improvement, at least when using back-projection algorithms for the reconstruction.
Journal ArticleDOI
The Reconstruction of a Three-Dimensional Structure from Projections and its Application to Electron Microscopy
TL;DR: In this paper, the Fourier transform is used to combine data from a number of different views of a transmission electron micrograph to reconstruct a 3D image of a given particle to a given degree of resolution.
Journal ArticleDOI
Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6
Tim Grant,Nikolaus Grigorieff +1 more
TL;DR: A method of using optimal exposure values to filter movie frames, yielding images with improved contrast that lead to higher resolution reconstructions that should benefit cryo-EM work on all types of samples, especially those of relatively low-molecular mass.
Book
Electron tomography : methods for three-dimensional visualization of structures in the cell
TL;DR: The principles of electron microscopy have been discussed in this article, including the use of the Electron Microscope as a structure projector, and the role of the Markerless Alignment in Electron Tomography.